LDH Cytotoxicity Assay Kit (CAT#: CB-P018-K) Datasheet

Lactate dehydrogenase (LDH) is an enzyme widely present in the cytoplasm of many organisms. Since LDH is a fairly stable enzyme, it has been widely used to assess tissue and cell damage and toxicity. The quantitative analysis of LDH has a wide range of applications. The enzyme measurement can be used to estimate biomass, count cells, and determine the health of cells and tissues. LDH is widely used for cytotoxicity testing and viability testing of cultured cells.

The LDH cytotoxicity assay kit provides a sensitive, reproducible and easy-to-use colorimetric method for cytotoxicity detection. The assay kit uses methyl dye INT as a colorimetric indicator of enzyme activity. LDH strips electrons from lactic acid and transfers them to NAD to produce NADH. Subsequently, NADH lowers the INT, which causes the color to change from yellow to red. The colorimetric reaction can be measured at 490nm.

•High-throughput screening
•Colorimetry: Measure the release of LDH by forming colored products.
•Fast and easy to use: simple step by step.
•Powerful: Stable LDH enzyme activity as a cytotoxic marker.
•Safety: No radioactivity.

For rapid determination of cytotoxicity based on lactate dehydrogenase released into the cell culture medium.
Evaluate toxic compounds, toxins, detergents, environmental pollutants, and physical treatment of cell lysis.