LDH Cytotoxicity Assay Kit (CAT#: CB-P018-K) Datasheet

Description
Lactate dehydrogenase (LDH) is an enzyme widely present in the cytoplasm of many organisms. Since LDH is a fairly stable enzyme, it has been widely used to assess tissue and cell damage and toxicity. The quantitative analysis of LDH has a wide range of applications. The enzyme measurement can be used to estimate biomass, count cells, and determine the health of cells and tissues. LDH is widely used for cytotoxicity testing and viability testing of cultured cells.

The LDH cytotoxicity assay kit provides a sensitive, reproducible and easy-to-use colorimetric method for cytotoxicity detection. The assay kit uses methyl dye INT as a colorimetric indicator of enzyme activity. LDH strips electrons from lactic acid and transfers them to NAD to produce NADH. Subsequently, NADH lowers the INT, which causes the color to change from yellow to red. The colorimetric reaction can be measured at 490nm.
Features
•High-throughput screening
•Colorimetry: Measure the release of LDH by forming colored products.
•Fast and easy to use: simple step by step.
•Powerful: Stable LDH enzyme activity as a cytotoxic marker.
•Safety: No radioactivity.
Applications
For rapid determination of cytotoxicity based on lactate dehydrogenase released into the cell culture medium.
Evaluate toxic compounds, toxins, detergents, environmental pollutants, and physical treatment of cell lysis.
Size
400/2,000 Assays
Sample
Cell culture (adherent and non adherent)
Species Reactivity
Mammalian
Components
20 mL LDH Assay Buffer
4 mL Lysis solution
20 mL Stop solution
Shipping
Gel Pack
Storage
LDH Assay Buffer (-20 °C) and Lysis Solution & Stop solution (+4°C).
Protect the product from light.
Shelf Life
24 months from the date of purchase, if it is properly stored.
Research Use
For Research Use Only! Not For Use in Humans.

All products and services are for Research Use Only. Do Not use in humans.

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