Anti-Human Properdin Monoclonal Antibody (clone 1G6D2) -10 mg (CAT#: CB-P449-AB) Datasheet

Antibody

Monoclonal antibody 1G6D2 recognizes human properdin, also known as complement factor P. The complement system is the first line of defense against pathogens and helps eliminate apoptosis and damaged cells. The positive regulator, plasma protein properdin, The alternative pathway of complement is essential. A single-chain glycoprotein (ca 53 kDa) composed of six TSR sequences, exists in the blood as a preferred head-to-tail trimer, dimer and tetramer mixture The protein is composed of leukocytes (such as activated neutrophils, monocytes, and T lymphocytes, for example, can also pass through stressed endothelial cells.
Properdin can start with C3 and factors B, D, I and H and actively regulate the activity of the alternative pathway. It binds to C3b, thereby stabilizing the unstable C3bBb convertase deposited on the immune complex or foreign surface. Thus, AP can be enhanced by stimulating the amplification of C3bBb convertase, which competes with factor I catabolism, and factor I uses factor H as a cofactor. The local amplification process leads to the production of the alternative pathway C5 convertase C3bBb3b and initiates the terminal pathway of complement activation. The alternative pathway can account for about 80% of the terminal pathway activity. Properdin has also shown the ability to directly limit the activity of factor H.
Recent studies have shown that properdin is also a pattern recognition receptor (PRR) that can directly bind to the surface of microorganisms, apoptotic and necrotic cells (dangerous non-self and altered self). Inappropriate activation or dysregulation of alternative pathways is a key factor in the development of several autoimmune diseases. Targets opsonized with properdin are marked as cleared by scavenger cells, even without complement. This makes it a potential target for disease treatment. Recent studies have shown that people have renewed interest in the role of properdin in the pathogenesis of diseases such as asthma, arthritis, septic shock, age-related macular degeneration (AMD) and C3 glomerulopathy.
Antibody 1G6D2 can be used in ELISA, Western blotting (unreduced only), and as a negative control antibody for functional studies to inhibit alternative pathways of complement.

Autoimmunity, Nephrology