Description
Annexin V cell apoptosis detection kit is a convenient, easy-to-use and safe cell apoptosis detection method. Annexins are a family of calcium-dependent phospholipid binding proteins that bind to phosphatidylserine (PS).
The externalization of phosphatidylserine residues on the outer plasma membrane of apoptotic cells can be detected by annexin V. Once the apoptotic cells are bound to the labeled Annexin V, they can be observed by fluorescence microscopy or cell counting.
Since the loss of membrane integrity is a pathological feature of necrotic cell death, necrotic cells will be stained with specific membrane-impermeable nucleic acid dyes (such as propidium iodide). The membrane integrity of apoptotic cells can be proved by excluding these dyes .
Applications
Detect early/middle stages of apoptosis; differentiate apoptosis from necrosis.
Size
100 Assays
Detection Method
Flow cytometry (Ex = 488 nm; Em = 530 nm) and fluorescence microscopy.
Sample
Works with both adherent and suspension cells.
Protocol Length
Get results in 9 minutes.
Components
Labeled Annexin V:500 µL
Binding Buffer (10x):50 mL
Propidium Iodide:1 mL
Shipping
Gel Pack
Storage
-20°C
Shelf Life
24 months from the date of purchase, if it is properly stored.
Research Use
For Research Use Only! Not For Use in Humans.
