Product Type
Antibody
Description
The monoclonal antibody M81 reacts with an epitope on C1 (a subcomponent of the first component of C (C1)) activated by human protein C. Activated C1s is a glycosylated single-peptide zymogen with a molecular weight of 85 kD. The activation of the protease C1s occurs through the cleavage of the active form of C1r. The activated protease (activated C1s) is composed of H and L chains connected by disulfide bonds. Activated C1s is a serine protease whose catalytic site is located in the L chain. The activation of the classical C pathway is triggered by activated C1s that cleave C4 and C2 to form the C3 convertase C4bC2a. The epitope recognized by antibody M81 is the IV and/or V domain of the activated C1sγ domain. The monoclonal antibody M81 prevents the activation of C4 and the binding of C4 to activated C1s. The antibody reacts near the binding site of C1s and reacts with active and inactive C1s.
Disease
Autoimmunity, Infectious diseases
Size
100 µg
Species
Human
Clonality
Monoclonal
Clone Number
M81
Buffer
PBS containing 0.1% bovine serum albumin.
Application
Flow cytometry, Frozen sections, Functional studies, Immuno assays, Immuno precipitation, Paraffin sections, Western blot
Application Notes
For immunohistology, the flow cytometer and Western blot diluent that will be used depend on the detection system used. It is recommended that users test reagents and determine their own optimal dilution. The typical initial working dilution is 1:10. In order to inhibit C1 from activating C4, it must be diluted according to the amount of C1 to be inactivated.
Storage Instructions
Product should be stored at 4°C. Under recommended storage conditions, product is stable for at least one year.
Alternative Names
complement C1s; C1s
