Fc Engineering Service for Effector Function Inhibition

Today, monoclonal immunoglobulin (IgG) antibodies have become the main choice for cancer treatment. The efficacy of monoclonal antibodies (mAbs) is achieved through their antigen-binding fragments (Fab) and crystallizable fragments (Fc). Fab can specifically recognize the tumor-associated antigen (TAA), thereby regulating the downstream signaling pathway connected by TAA, which may lead to the inhibition of tumor growth and induce tumor cell apoptosis and differentiation. The Fc region can further improve the efficacy of mAbs by mediating effector functions such as antibody-dependent cell cytotoxicity (ADCC), complement-dependent cytotoxicity (CDC), and antibody-dependent cell phagocytosis (ADCP). In view of the fact that the Fc region plays a vital role in regulating the efficacy of mAbs in cancer treatment, extensive research on Fc engineering has been conducted in recent years.

Creative Biolabs focuses on the recent progress in therapeutic Fc engineering-associated effector functions (ADCC, ADCP, and CDC), and are committed to developing the optimal Fc mutation strategy to support the innovative drugs currently being studied in clinical trials, and fine-tune them to achieve the required treatment effect.

FcγRIIb functions in inhibiting the immune system

The FcγR composed of FcγRI (CD64), FcγRII (CD32) and FcγRIII (CD16) are heterogeneous in terms of cell expression and Fc binding affinity. FcγRI binds to the Fc region with KD~10-8-8-10-9 M, and is expressed in monocytes, dendritic cells and IFN-γ-activated neutrophils. FcγRII binds to the Fc region with a relatively low affinity (KD~10-7 M) and exists in five isoforms. Among them, the inhibitory factor FcγRIIb, which mainly carries an inhibitory motif based on immunoreceptor tyrosine on B lymphocytes, is essential for immune regulation.

FcγRIIb is the only Fc receptor with inhibitory function. It is usually expressed on the same cells as other activating FcγRs, and plays a negative feedback regulation effect on the signals of FcγRIIa and FcγRIIIa. Therefore, FcγRIIb can be applied to the treatment of autoimmune diseases and allergic diseases. In addition, FcγRIIb is the only Fc receptor expressed on B cells, so antigen-specific B cells can recognize the complex of self-antigen and therapeutic antibody through FcγRIIb, thereby exerting an immunosuppressive effect. Therefore, FcγRIIb can be used as a key checkpoint of humoral immune response and play a role in diseases such as lupus erythematosus (SLE).

Strategies to regulate the immune response mediated by FcgR. Fig.1 Strategies to regulate the immune response mediated by FcgR. (Sondermann, 2016)

By manipulating the recruitment of activated and inhibitory receptors, such as through engineered antibodies, a treatment plan can be tailored to the optimal treatment for a specific indication.

Highlight clinical trials of engineered Fc to inhibit the immune system

Studies have found that Fc modifications such as S267E/L328F or P238D can increase the affinity of Fc and FcγRIIb by more than 100 times. XmAb5871 is a humanized antibody. Its Fc region has been modified by S267E/L328F to enhance its affinity for FcγRIIb. It activates FcγRIIb through its anti-CD19Fab region, thereby inhibiting the activation of B cells without causing cytotoxicity to B cells. It is currently in a phase I clinical trial for the treatment of SLE. The anti-CD19 Fab fragment in XmAb5871 is exactly the same as the anti-CD19 Fab in XmAb5574 mentioned above, but the two amino acid differences in the Fc region lead to their different functions. In addition, another anti-IgE antibody XmAb7195 is also modified with S267E/L328F to enhance its affinity for FcγRIIb. It can not only neutralize soluble IgE, but also selectively inhibit the activation of IgE+B cells. XmAb7195 is currently in Phase I clinical treatment of allergic diseases.

Fc Engineering Isotype Intended Function Result
S267E/L328F Glyco-IgG1 Higher affinity to FcγRIIb Reduced effector function
E233D/G237D/H268D/P271G/Y296D/A330R Glyco-IgG1 Higher affinity to FcγRIIb
P238D/L328E Glyco-IgG1 Higher affinity to FcγRIIb
L235E IgG2 Reduced FcγR and C1q binding Reduced CDC
IgG2/IgG4 cross isotype IgG2/IgG4 Reduced FcγR and C1q binding
IgG2: H268Q/V309L/A330S/P331S IgG2 Reduced FcγR and C1q binding
IgG2: V234A/G237A/P238S/H268A/V309L/
A330S/P331S
IgG2 Reduced FcγR and C1q binding

Fig.2 Fc modification for suppressing the immune system.

For more detailed information, please feel free to contact us or directly sent us an inquiry.

Reference

  1. Sondermann, P., & Szymkowski, D. E. (2016). Harnessing Fc receptor biology in the design of therapeutic antibodies. Current opinion in immunology, 40, 78-87.

RESOURCES

Creative Biolabs provides luciferase-based ADCC assay. This Jurkat cell based assay is pioneered by Creative Biolabs, and the methodology is very well accepted by the field. See attached ADCC Reporter Assay Protocol for further details. 

All products and services are for Research Use Only. Do Not use in humans.

CASE STUDY

ADCC Assay WT vs AfucoTM Mabs  Visit

Resource

Antibody Fc Engineering: Towards Better Therapeutics  Visit

ONLINE INQUIRY

Creative Biolabs has established a team of customer support scientists ready to discuss ADCC/CDC optimization strategies, antibody production, bioinformatics analysis and other molecular biology/biotechnology issues.

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