Fc-Gamma Receptor Binding Assay Services

Fcγ receptors are a family of glycoproteins expressed on a variety of immune cells and have affinity for the Fc region of immunoglobulin G (IgG). The receptor family includes several members: FcγRI (CD64), FcγRIIa/b/c (CD32a/b/c) and FcγRIIIa/b (CD16a/b). Each of them has a characteristic preference for binding to the IgG isotype and can transduce activation signals (for example, FcγRI, IIa, IIIa) or inhibitory signals (for example, FcγRIIb). FcγR has been shown to be very important in regulating the efficacy of therapeutic antibodies through FcγR-mediated phagocytosis, cytokine production, antibody-dependent cell-mediated cytotoxicity (ADCC) and antibody-dependent cell-mediated phagocytosis (ADCP).

Creative Biolabs provide FcR binding assays to obtain detailed kinetic information about antibody-receptor interactions. Two different measurement settings are established in opposite coupling directions to select the best measurement for your research.

Custom antibody binding assay development

Creative Biolabs perform FcγR binding assays for various IgG antibody isotypes or Fc fusion proteins. This system is a universal label-free system with excellent sensitivity. Our team of scientific experts will work with you to optimize the performance of the FcγR binding assay, and apply this experience to novel antibody therapies to perform assays in a variety of different formats to meet your needs, including:

• FcγR1/ CD64
• FcγR2a/ CD32a R variant
• FcγR2a/ CD32a H variant
• FcγR2b/ CD32b
• FcγR3a/ CD16a V variant
• FcγR3a/ CD16a F variant
• FcγR3b/ CD16b

Fig.1 The family of human FcγRs.^{1, 2}

Working with Creative Biolabs

Creative Biolabs conducts bioassays on behalf of clients, either as a standalone project or as part of a larger development project. We focus on developing the correct bioassays to help you understand the molecule and help guide you correctly. With extensive experience in Fc characterization, our team can work on custom solutions to meet the unique requirements of each product. With flexible global capabilities, you can provide services where you need it most.

Our offer includes:

• Use appropriate coupling strategies to capture antibodies or Fc receptors
• Qualitative and quantitative kinetic analysis of FcγR/ FcRn interaction, providing rate constants for association and dissociation and affinity (ka, kd, KD)

If you are interested in conducting SPR research with us and get more information, work plans and quotations, please contact us our expert scientists today to discuss how our range of Fc analytical services can improve your unique process.

Creative Biolabs provides a complete collection of recombinant Fc receptor proteins, including their common variants, to help you speed up the development of mAbs. Please visit: Fc Receptor Proteins

References

1. Vogelpoel, Lisa TC, et al. "Control of cytokine production by human fc gamma receptors: implications for pathogen defense and autoimmunity." Frontiers in Immunology 6 (2015): 79.
2. under Open Access License CC BY 4.0, without modification.