Fcγ receptors are a family of glycoproteins expressed on a variety of immune cells and have affinity for the Fc region of immunoglobulin G (IgG). The receptor family includes several members: FcγRI (CD64), FcγRIIa/b/c (CD32a/b/c) and FcγRIIIa/b (CD16a/b). Each of them has a characteristic preference for binding to the IgG isotype and can transduce activation signals (for example, FcyRI, IIa, IIIa) or inhibitory signals (for example, FcyRIIb). FcγR has been shown to be very important in regulating the efficacy of therapeutic antibodies through FcγR-mediated phagocytosis, cytokine production, antibody-dependent cell-mediated cytotoxicity (ADCC) and antibody-dependent cell-mediated phagocytosis (ADCP).
Creative Biolabs uses the Biacore T200 instrument to provide FcR binding assays to obtain detailed kinetic information about antibody-receptor interactions. Two different measurement settings are established in opposite coupling directions to select the best measurement for your research.
Creative Biolabs uses Biacore T200 and 4000 systems to perform FcγR binding assays for various IgG antibody isotypes or Fc fusion proteins. This system is a universal label-free system with excellent sensitivity. Our team of scientific experts will work with you to optimize the performance of the FcγR binding assay, and apply this experience to novel antibody therapies to perform assays in a variety of different formats to meet your needs, including:
Creative Biolabs conducts bioassays on behalf of clients, either as a standalone project or as part of a larger development project. We focus on developing the correct bioassays to help you understand the molecule and help guide you correctly. With extensive experience in Fc characterization, our team can work on custom solutions to meet the unique requirements of each product. With flexible global capabilities, you can provide services where you need it most.
Our offer includes:
If you are interested in conducting SPR research with us and get more information, work plans and quotations, please contact our expert scientists today to discuss how our range of Fc analytical services can improve your unique process.
Creative Biolabs provides a complete collection of recombinant Fc receptor proteins, including their common variants, to help you speed up the development of mAbs. Please visit: Fc Receptor Proteins
Creative Biolabs provides luciferase-based ADCC assay. This Jurkat cell based assay is pioneered by Creative Biolabs, and the methodology is very well accepted by the field. See attached ADCC Reporter Assay Protocol for further details.
All products and services are for Research Use Only. Do Not use in humans.
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Creative Biolabs has established a team of customer support scientists ready to discuss ADCC/CDC optimization strategies, antibody production, bioinformatics analysis and other molecular biology/biotechnology issues.