The immense diversity and complexity of glycan structures and their multiple modes of interaction with proteins pose great challenges to the development of therapeutic antibodies. Various tools for glycan profiling based on chromatography, mass spectrometry, glycan microarray and glycoinformatics are being developed. Lectins, which have long been used in glycoimmunology, are printed on microarrays, providing a universal platform for rapid high-throughput analysis of glycoforms in biological samples. Lectin microarray technology surpasses traditional mass spectrometry technology for glycomic analysis in both efficiency and function. At Creative Biolabs, we can offer the comprehensive lectin microarray analysis services, which can analyze the binding modes of a variety of glycoforms, while being fast, simple and requiring the least amount of sample.
We selected 26 lectins to be included in our first-generation lectin microarray. These 26 lectins represent most of the epitopes that bind to carbohydrates and have been characterized on our glycan array. There are 8 or 16 identical sub-arrays on a single array chip, so 8 or 16 samples can be analyzed simultaneously. Our lectin microarrays provide scientists with powerful and sensitive tools for analyzing the glycosylation profiles of therapeutic proteins, biomarkers or other proteins of interest.
Lectin microarrays have been prepared using multiple immobilization techniques (1) using lectins derived from multiple sources (2). The binding of glycoconjugates (3) of analytes from multiple biological samples has been tested using multiple detection methods (4). Lectin microarrays provide fast, sensitive and high-throughput screening tools for sugar profile analysis, which can be used for important biomedical applications (5). The simultaneous application of lectin microarray and complementary glycomics technology has the potential to decode the "glycosyl code" and develop new effective diagnostic and therapeutic methods.
Fig.2 Workflow for lectin microarray technologies.
After the data is generated and processed, the customer will receive:
If additional fees are required, customers can also get a difference analysis report, which contains difference analysis (cluster analysis, ROC analysis) and an explanation of the difference in glycan structure between samples.
Creative Biolabs provides luciferase-based ADCC assay. This Jurkat cell based assay is pioneered by Creative Biolabs, and the methodology is very well accepted by the field. See attached ADCC Reporter Assay Protocol for further details.
All products and services are for Research Use Only. Do Not use in humans.
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Creative Biolabs has established a team of customer support scientists ready to discuss ADCC/CDC optimization strategies, antibody production, bioinformatics analysis and other molecular biology/biotechnology issues.