FcRn Binding Assay Services

The key factor in the efficacy of therapeutic antibodies is the serum half-life, which largely depends on the interaction between the antibody and the neonatal Fc receptor (FcRn). FcRn is a class I MHC molecule related to β-2-microglobulin, which prevents the degradation of IgG, thereby prolonging the half-life in vivo. The functions of FcRn include bidirectional IgG-mediated phagocytosis across cell membranes and enhancement of antigen presentation. The binding affinity of FcRn to IgG is negligible at physiological pH, and binding interactions can only occur after the early endosomes undergo endocytosis. The IgG/FcRn complex can then be returned to the cell surface. Therefore, evaluating and understanding the ability of antibody products to bind FcRn plays a pivotal role in the development of safe and effective molecules.

Fig.1 Emerging roles of FcRn in cancer.^{1, 2}

Flexible FcRn Binding Services

Creative Biolabs provides ready-to-use FcRn binding assay based on our advanced platform, which is a versatile, label-free system with excellent sensitivity. Our method has been extensively validated using commercially available mAbs, and we work with you to validate or validate your materials to ensure the success of the process.

We also provide FcRn binding to the most common toxicology species to help you make decisions and interpretations in toxicology research. The binding of cynomolgus monkey, rat and mouse FcRn to human IgG or fusion protein can be demonstrated and compared with the binding to human FcRn. Our experience in method development and qualification makes Creative Biolabs your perfect support from clone ranking and drug candidate selection to the clinical stage.

Working with Creative Biolabs

Creative Biolabs conducts bioassays on behalf of clients, either as a standalone project or as part of a larger development project. We focus on developing the correct bioassays to help you understand the molecule and help guide you correctly. With extensive experience in Fc characterization, our team can work on custom solutions to meet the unique requirements of each product. With flexible global capabilities, you can provide services where you need it most.

Contact our expert scientists today to discuss how our range of Fc analytical services can improve your unique process.

References

1. Pyzik, Michal, et al. "The neonatal Fc receptor (FcRn): a misnomer?." Frontiers in Immunology 10 (2019): 1540.
2. under Open Access License CC BY 4.0, without modification.