Creative Biolabs has been committed to the continuous exploration and development of ADCC research. We have formed a complete service system to help you accelerate the research of projects in this field. In this module, we share with you some knowledge about the progress of ADCC research to help you better understand.
ADCP is mediated by monocytes, macrophages, neutrophils, and dendritic cells to engulf disease cells by expressing FcγRIIa (CD32a), FcγRI (CD64), and FcγRIIIa (CD16a), and blockade studies have shown that FcγRIIa is the primary FcγR receptor involved in this process. Studies have shown that the effects of ADCP are enhanced when treated with immunomodulators. Therefore, ADCP is of great significance in the research and development of antibody drugs. Please click on the keywords to learn more.
Complement is a group of enzymatically active proteins found in human or vertebrate serum and tissue fluids, including more than 30 soluble and membrane-bound proteins. The antibody first binds to complement C1q, and then C2-C9 is activated to form an attack complex that exerts a lytic effect on the target cells. Many antitumor antibodies can cause CDC effects, such as CD20 antibody, CD52 antibody, and CEA antibody. Please click on the keywords to learn more.
Among the four common IgG isoforms, IgG1 and IgG3 can induce strong ADCC effects, but IgG1 has a longer half-life and a more stable structure than IgG3, so ADCC effector antibodies usually choose the IgG1 isoform. To exert the ADCC effect, the antibody needs to bind to the Fc receptor (FcγR). Antibodies typically bind to FcγR through the hinge region and the CH2 region. The Asn-N297-linked glycosylation of the CH2 domain and the modified glycoform components (such as acetylglucosamine, mannose, fucose, and sialic acid) determine the stability and binding properties of CH2 structure. Therefore, ADCC effect antibody and Fc segment sugar modification are the main engineering processes. Please click on the keywords to get more relevant knowledge.
Based on the important role of ADCC in the research and development of antibody drugs, it is hoped that the activation effect of ADCC can be improved by changing the design of antibodies, and the main way is to improve the binding ability of Fc to FcγR, which is achieved through amino acid mutation and glycosylation modification in technical strategy. Therefore, the detection of ADCC is inseparable from the biological activity analysis and quality attribute control of therapeutic antibody drugs. Please click on the keywords to get more relevant knowledge.
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