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CreaClean™ Post-Exoglycosidase Clean-up Spin Columns [For Glycan Clean Up] (CAT#: CB-P283-K) Datasheet

Product Type
Kit
Description
The spin column contains a special modified polyethersulfone membrane with a molecular weight of approximately 30 kDa.
Features
Number of Samples: Each pack contains 6 spin columns
Amount of Sample: Up to 500 µL per column
Centrifugal Force Up to 14,000 x g
Suitable Samples: Unlabelled and fluorophore labelled (e.g. 2-AB, 2-AA or procainamide labelled) glycans
released from glycoproteins or other sources and treated with exoglycosidase
enzymes.
Applications
Post-exoglycosidase Clean-up
For removal of exoglycosidase enzymes and other protein material following glycan enzymatic digestion/ sequencing. This will prevent contamination of HPLC columns during subsequent chromatographic analysis. The spin columns can also be used to remove exoglycosidases or other proteins before mass spectrometry analysis of glycans.
Sample
Glycoproteins and glycopeptides containing N-linked glycans
Components
CreaClean Post-Exoglycosidase Clean-up Spin Columns With Sample Collection Vials 6
Handling Advice
Ensure that any glass, plastic ware or solvents used with this item are free of environmental carbohydrates. Use powder-free gloves for all sample handling procedures and avoid contamination with environmental carbohydrate. once used, the plate should be discarded according to local safety rules
Storage
Store at room temperature. Protect from sources of heat, light, and moisture. When stored correctly, the reagents should be stable for 36 months from date of purchase.
Shelf Life
Stability: Stable at least 12 months when stored properly. Several days exposure to ambient tempertures will not reduce activity.
Research Use
For research use only. Not for human or drug use

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All products and services are for Research Use Only. Do Not use in humans.

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