Permethylated glycan library [Permethylated Glycan Standards] (CAT#: CB-P322-K) Datasheet

Glycan Standards

Structural analysis of carbohydrates is a requirement for biopharmaceutical characterization, and may include the assay of the following characteristics: molecular weight, composition of monosaccharides and their configuration and conformational isomers, sequence of monosaccharide residues, presence and location of branches and functional groups And the relationship between glycosidic bonds. Mass spectrometry (MS) is a useful tool for determining molecular weight, but the sensitivity may be low, especially when there are many different structures. Carbohydrates usually contain carboxyl, amino, sulfate and phosphate. The nature and location of these groups on the residue, and the location of the residue in the glycan may be difficult to determine. Permethylation of glycans converts hydrogen groups into methyl groups, thereby making the glycans hydrophobic. This conversion stabilizes sialic acid and can inCBLse the signal intensity in quality measurement. Now, CBLtive Biolabs provides a library of fully methylated IgG glycans that can be used as a system suitability standard during MS analysis. CBL also provides a C13 version, which can be used as an internal standard in the same MALDI chip spot as your C12 labeled permethylated glycan.