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Glucose-6-Phosphate Assay Kit (Fluorometric) - High Sensitivity (CAT#: CB-P177-K) Datasheet

Product Type
Kit
Description
Creative Biolabs' Glucose 6-Phosphate Assay Kit is a quantitative, simple, sensitive and rapid that can quantitatively analyze glucose 6-phosphate (G6P) in various samples. In the measurement, glucose 6-phosphate is oxidized to produce a product (Ex / Em 535/587 nm) that converts the almost colorless probe into a strongly fluorescent product. Assuming a 5-fold dilution during processing, this assay can detect G6P ranging from 10 to 500 pmoles, which is equivalent to the range of 1-500 µM in the original sample.
Applications
Functional Studies
Size
1 kit
Detection Method
Colorimetric/Fluorometric
Detection Limit
1 µM - 500 µM
Sensitivity
> 1 µM
Sample
Tissue, Adherent cells, Suspension cells, Tissue Homogenate
Species Reactivity
Mammals
Protocol Length
0h 40m
Components
Glucose 6 phosphate assay buffer 1 x 25ml
Glucose 6 phosphate Enzyme Mix (lyophilized) 1 vial
Glucose 6 phosphate Standard (10 µmol; lyophilized) 1 vial
Glucose 6 phosphate Substrate Mix (lyophilized) 1 vial
Probe (in DMSO) 1 x 0.4ml
Requires
Microplate reader
Storage
Please refer to protocols.
Shelf Life
12 months
Research Use
For research use only. Not for human or drug use

All products and services are for Research Use Only. Do Not use in humans.

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