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Glucose-6-Phosphate Assay Kit (Fluorometric) - High Sensitivity (CAT#: CB-P177-K) Datasheet

Product Type
Kit
Description
Creative Biolabs' Glucose 6-Phosphate Assay Kit is a quantitative, simple, sensitive and rapid that can quantitatively analyze glucose 6-phosphate (G6P) in various samples. In the measurement, glucose 6-phosphate is oxidized to produce a product (Ex / Em 535/587 nm) that converts the almost colorless probe into a strongly fluorescent product. Assuming a 5-fold dilution during processing, this assay can detect G6P ranging from 10 to 500 pmoles, which is equivalent to the range of 1-500 µM in the original sample.
Applications
Functional Studies
Size
1 kit
Detection Method
Colorimetric/Fluorometric
Detection Limit
1 µM - 500 µM
Sensitivity
> 1 µM
Sample
Tissue, Adherent cells, Suspension cells, Tissue Homogenate
Species Reactivity
Mammals
Protocol Length
0h 40m
Components
Glucose 6 phosphate assay buffer 1 x 25ml
Glucose 6 phosphate Enzyme Mix (lyophilized) 1 vial
Glucose 6 phosphate Standard (10 µmol; lyophilized) 1 vial
Glucose 6 phosphate Substrate Mix (lyophilized) 1 vial
Probe (in DMSO) 1 x 0.4ml
Requires
Microplate reader
Storage
Please refer to protocols.
Shelf Life
12 months
Research Use
For research use only. Not for human or drug use

Figure 1 Glucose 6 phosphate measured in mouse tissue lysates showing quantity (pmol) per mg protein of tested sample

Figure 2 Glucose 6 phosphate measured in biological fluids showing quantity (pmol) per microliter of tested sample.

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All products and services are for Research Use Only. Do Not use in humans.

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