Product Type
Kit
Description
Ceramidoglycanase is an enzyme used to release glycans from glycosphingolipids (GSL) for their characterization. It cleaves glycans including GM1, GM2 and GM3 by cleaving β-glycosyl bonds. The sugar can then be labeled using CreaTag labeling technology.
Features
Source: Hirudo medicinalis
Number of Samples: Sufficient for up to 25 samples
Amount of Sample:s: Up to 1 nmol of glycolipid
Suitable Samples: Isolated glycolipids containing a ceramide backbone
Applications
Ceramide glycanase deglycosylates a variety of glycosphingolipids by cleaving the β-glycosyl linkage.
Size
55 µL; Sufficient for up to 150 reactions.
Sample
Glycoproteins and glycopeptides containing N-linked glycans
Components
CreaZyme Ceramide Glycanase - 1 vial of 55 µL
RXN buffer (4x concentrate) - 1 vial of 150 µL
GM1 glycolipid - 1 vial of 10 µL (approx. 2 nMol)
Storage
Long term storage - over 14 days - store at - 20°C.
Protect from sources of heat and light. Avoid multiple freeze thaw cycles.
Short term storage - up to 14 days - store at 4°C.
Shelf Life
Stability: Stable at least 12 months when stored properly. Several days exposure to ambient tempertures will not reduce activity.
Research Use
For research use only. Not for human or drug use
