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CreaZyme™ Ceramide Glycanase Kit [For Glycan Release] (CAT#: CB-P262-K) Datasheet

Product Type
Ceramidoglycanase is an enzyme used to release glycans from glycosphingolipids (GSL) for their characterization. It cleaves glycans including GM1, GM2 and GM3 by cleaving β-glycosyl bonds. The sugar can then be labeled using CreaTag labeling technology.
Source: Hirudo medicinalis

Number of Samples: Sufficient for up to 25 samples

Amount of Sample:s: Up to 1 nmol of glycolipid

Suitable Samples: Isolated glycolipids containing a ceramide backbone
Ceramide glycanase deglycosylates a variety of glycosphingolipids by cleaving the β-glycosyl linkage.
55 µL; Sufficient for up to 150 reactions.
Glycoproteins and glycopeptides containing N-linked glycans
CreaZyme Ceramide Glycanase - 1 vial of 55 µL
RXN buffer (4x concentrate) - 1 vial of 150 µL
GM1 glycolipid - 1 vial of 10 µL (approx. 2 nMol)
Handling Advice
1. Pipette 5 µL of LZ-CER-BUFFX4 into an Eppendorf tube, followed by 5 µL of approximately 1nmol of the glycolipid sample to be tested.
2. For the positive control reaction, add 5 µL of GLIP-GM1-01 substrate to 5 µL of LZ-CER-BUFFX4 into an Eppendorf tube
3. Add 2 µL of LZ-CER-HM-10 (the enzyme) to the reaction mixture and mix gently by tapping the Eppendorf tube with your finger. Make total reaction mix up to 20 µL with water.
4. Incubate your samples in a water bath, oven or any other constant heating source at 37°C for 24 hrs.
Long term storage - over 14 days - store at - 20°C.
Protect from sources of heat and light. Avoid multiple freeze thaw cycles.
Short term storage - up to 14 days - store at 4°C.
Shelf Life
Stability: Stable at least 12 months when stored properly. Several days exposure to ambient tempertures will not reduce activity.
Research Use
For research use only. Not for human or drug use

All products and services are for Research Use Only. Do Not use in humans.


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