55 µL; Sufficient for up to 150 reactions.

Glycoproteins and glycopeptides containing N-linked glycans

CreaZyme Ceramide Glycanase - 1 vial of 55 µL
RXN buffer (4x concentrate) - 1 vial of 150 µL
GM1 glycolipid - 1 vial of 10 µL (approx. 2 nMol)

1. Pipette 5 µL of LZ-CER-BUFFX4 into an Eppendorf tube, followed by 5 µL of approximately 1nmol of the glycolipid sample to be tested.
2. For the positive control reaction, add 5 µL of GLIP-GM1-01 substrate to 5 µL of LZ-CER-BUFFX4 into an Eppendorf tube
3. Add 2 µL of LZ-CER-HM-10 (the enzyme) to the reaction mixture and mix gently by tapping the Eppendorf tube with your finger. Make total reaction mix up to 20 µL with water.
4. Incubate your samples in a water bath, oven or any other constant heating source at 37°C for 24 hrs.

Long term storage - over 14 days - store at - 20°C.
Protect from sources of heat and light. Avoid multiple freeze thaw cycles.
Short term storage - up to 14 days - store at 4°C.

Stability: Stable at least 12 months when stored properly. Several days exposure to ambient tempertures will not reduce activity.

For research use only. Not for human or drug use