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Endoglycosidase F1 [For Glycan Release] (CAT#: CB-P252-K) Datasheet

Product Type
Kit
Description
Endo F1 cleaves high mannose and some hybrid N-glycans from peptides and proteins.
Endo F1 cleaves asparagine-linked high mannose and some hybrid oligosaccharides. Core fucosylation reduces the activity by 50 times. Endoglycosidase F1 will hydrolyze sulfates containing high mannose chains. It cleaves between the two N-acetylglucosamine residues in the diacetyl chitobiose core of the oligosaccharide to produce a truncated sugar molecule, one of which remains in the asparagus Amide on. In contrast, PNGase F completely eliminated oligosaccharides.
Features
Source: Recombinant Elizabethkingia miricola in E. Coli
EC: 3.2.1.96
Alternative Names: Endo F1, Endoglycosidase F1, endo-β-N-acetylglucosaminidase F
Endo F1 Specificity: Cleaves all asparagine-linked high mannose and some hybrid oligosaccharides
Specific Activity:>16 U/mg
Activity:>17 U/mL
Molecular weight: 32,000 daltons
Size
1 U / 60 µL
Sensitivity
Defined as the amount of enzyme required to catalyze the release of N-linked oligosaccharides from 1 micromole of denatured Ribonuclease B (RNase B) in 1 minute at 37°C, pH 5.5. Cleavage is monitored by SDS-PAGE (cleaved RNase B migrates faster).
Sample
Glycoproteins and glycopeptides containing N-linked glycans
Components
60 µL aliquot of enzyme (1 U) in 20 mM Tris-HCl, pH 7.5
5x Reaction Buffer - 250 mM sodium phosphate, pH 5.5
Handling Advice
1. Add up to 200 µg of glycoprotein to an Eppendorf tube. Adjust to 38 µL final volume with de-ionized water.
2. Add 10 µL 5x Reaction Buffer 5.5
3. Add 2.0 µL of Endo F1. Incubate 1 hour at 37°C.
Storage
Store enzyme at 4°C.
Shelf Life
Stability: Stable at least 12 months when stored properly. Several days exposure to ambient tempertures will not reduce activity.
Research Use
For research use only. Not for human or drug use

All products and services are for Research Use Only. Do Not use in humans.

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