NA3 Glycan (A3G3), 2-AB Labelled [Labeled Glycan Standards] (CAT#: CB-P327-K) Datasheet

Product Type
Glycan Standards
Description
Asialo-, tri-antennary complex-type N-glycan (oligosaccharide). NA3 is a the asialosubstructure of A3 glycan. The product provided may be a mixture of isomers differing in the linkage of the Gal residue of one of the glycan's antennae (please see the product certificate of analysis for lot specific details on the isomers provided). The different Gal linkages in NA3 are described in detail in the notes below.
Features
Sources: NA3 glyans are found on several mammalian glycoproteins including bovine serum fetuin (bSF). This product is typically purified from the oligosaccharide pool released from bSF by hydrazinolysis using a combination of HPLC and glycosidase digestion.
Form: Dry. Dried by centrifugal evaporation from an aqueous solution.
Molecular Weight: 2007
Purity:> 90% pure as assessed by a combination of 1 H-NMR and HPLC.
Sample
Glycoproteins and glycopeptides containing N-linked glycans
Handling Advice
Allow the unopened vial to reach ambient temperature and tap unopened on a solid surface to ensure that most of the lyophilized material is at the bottom of the vial. Gently remove the cap, add the desired volume of reconstitution medium, re-cap and mix thoroughly to bring all the oligosaccharide into solution. For maximal recovery of oligosaccharide, ensure that the cap lining is also rinsed and centrifuge the reconstituted vial briefly before use. Ensure that any glass, plasticware or solvents used are free of glycosidases and environmental carbohydrates.
Storage
Refridgerate (-20°C) both before and after dissolution. This product is stable for at least 5 years as supplied.
Shelf Life
Stability: Stable at least 12 months when stored properly. Several days exposure to ambient tempertures will not reduce activity.
Research Use
For research use only. Not for human or drug use

Figure 1 Structure

Figure 2 500 MHz 1H-NMR of NA3 Glycan

Figure 3 MS of NA3 Glycan

All products and services are for Research Use Only. Do Not use in humans.

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