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Glucose-6-Phosphate Assay Kit (Colorimetric) (CAT#: CB-P174-K) Datasheet

Product Type
Kit
Description
Glucose 6-phosphate content assay kit (colorimetric) is a simple, sensitive, and rapid test for quantifying the content of glucose 6-phosphate (G6P) in various samples. In this assay, glucose-6-phosphate is oxidized to an intermediate product, which in turn converts the probe to a dark product, which absorbs at OD = 450 nm. This product can detect G6P in the range of 1-30 nmoles, with a assay sensitivity of about 10 µM G6P.
Applications
Functional Studies
Size
1 kit
Detection Method
Colorimetric/Fluorometric
Detection Limit
1 µM - 30 µM
Sensitivity
10 µM
Sample
Plasma, Tissue, Adherent cells, Suspension cells, Tissue Homogenate
Species Reactivity
Mammals
Protocol Length
0h 40m
Components
G6P Assay Buffer 1 x 25ml
G6P Enzyme Mix (Lyophilised) 1 vial
G6P Standard (10 µmol) (Lyophilised) 1 vial
G6P Substrate Mix (Lyophilised) 1 vial
Requires
Microplate reader
Storage
Please refer to protocols.
Shelf Life
12 months
Research Use
For research use only. Not for human or drug use

Figure 1 Colorimetric standard curve: mean of duplicates (+/- SD) with background reads subtracted.

All products and services are for Research Use Only. Do Not use in humans.

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