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Superoxide Dismutase (SOD) Activity Assay Kit (CAT#: CB-P052-K) Datasheet

Description
Superoxide dismutase (SOD) catalyzes the decomposition of superoxide free radicals and provides the first line of defense against oxygen poisoning.

In the SOD analysis kit, xanthine oxidase (XO) converts xanthine and O2 into uric acid and H2O2 to produce superoxide ions. Then, the superoxide anion converts the tetrazolium salt WST-1 into the colored product WST-1 methyl maz. Then use a standard microplate reader to measure the absorbance at 450 nm. The addition of SOD to this reaction reduces the superoxide ion level, thereby reducing the rate of formation of WST-1 formaz. The SOD activity in the experimental samples was expressed as the percentage of inhibition of the formation rate of WST-1 formazan.
Features
Easy-to-use.
Fast protocol.
Applications
Quantitative determination of Superoxide Dismutase (SOD) enzyme activity.
Size
100 Assays
Detection Method
Absorbance (450 nm)
Sample
Whole blood, plasma, serum, urine, tissue and cell extracts
Species Reactivity
Mammalian
Components
1 mL WST-1 reagent
20 mL SOD Assay Buffer
10 mL SOD Dilution Buffer
30 μL SOD Enzyme solution
1 mg SOD Standard (300 U/mg)
Shipping
Gel Pack
Storage
The SOD Standard should be stored at €20 °C. All other components of this kit are stable at 4 °C until the kit expiration date.
Shelf Life
24 months from date of purchase, if stored properly.
Research Use
For Research Use Only! Not For Use in Humans.

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All products and services are for Research Use Only. Do Not use in humans.

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