Description
Kinases (also called phosphotransferases) constitute a large class of enzymes that transfer phosphate groups from the high-energy donor molecule ATP to its specific substrate. Kinases are known to regulate most cellular processes. The largest group in this family is protein kinases. To date, 518 different kinases have been identified in humans, and these kinases modify up to 30% of human proteins. The huge diversity and its key role in cell signaling make it an ideal target for drug development.
Creative Biolabs' Kinase Assay Kit provides a simple and fast method to determine kinase activity and high-throughput screening of kinase inhibitors. This microplate-based homogeneous assay involves incubating the kinase with a single working reagent, in which ADP is enzymatically converted into ATP and pyruvate, and quantified using a fluorescence method (530nm / 590nm).
Features
•Safety. Non-radioactive determination.
•Sensitive and accurate. It can quantify as low as 0.01 U/L kinase.
•Similar and convenient. Type determination of "mixed cultivation measures". The entire assay involves adding a working reagent and incubating for 10 minutes at room temperature.
•Rugged and suitable for HTS: The analytical method can tolerate up to 300 uM of ATP and 10% dimethyl sulfoxide (DMSO). It can be easily automated on the HTS liquid handling system, making thousands of measurements per day.
Applications
Fast and uniform protein kinase activity determination and high-throughput screening of kinase inhibitors.
Size
400 tests
Detection Method
FL530/585nm
Detection Limit
0.01 U/L
Sample
Cells etc
Protocol Length
10 min
Components
Reagent A: 10 mL Reagent B: 10 mL Assay Buffer: 25 mL Standard: 100 mL 3 mM ADP
Shipping
Gel Pack
Storage
Store all reagents at -20°C
Shelf Life
6 months
Research Use
For Research Use Only! Not For Use in Humans.
