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Neutral Red Cell Cytotoxicity Assay Kit (CAT#: CB-P020-K) Datasheet

Neutrophil cytotoxicity assay is one of the commonly used methods for detecting cell viability or drug cytotoxicity. The principle of this assay is based on the detection of living cells by uptake of the dye neutral red. Neutral Red is a eukaryotic dye that can stain lysosomes in living cells. Living cells can absorb neutral red through active transport and incorporate the dye into their lysosomes, but non-living cells cannot absorb the chromophore. Therefore, after washing, living cells can release the incorporated dye under the conditions of acidification extraction. The amount of dye released can be used to determine the total number of living cells or drug cytotoxicity. The neutral red absorption assay provides a quantitative measurement of the number of viable cells, which can be measured at OD 540 nm.

Creative Biolabs' neutrophil cytotoxicity test kit is simple, accurate and reproducible. It also includes doxorubicin as a positive control. This kit provides an excellent and effective method for in vitro cytotoxicity research and high-throughput drug screening, and each well can detect 5,000-50,000 cells.
•Simple procedure; takes less than 2 hours
•Fast, sensitive and convenient
This assay provides a convenient and non-radioactive alternative to performing cytotoxicity assays.
This assay relies on the ability of neutrophils to stain live cells' lysosomes.
1000 assays
Detection Method
Absorbance (540 nm)
Cell culture (adherent and non adherent)
Species Reactivity
100X Neutral Red Staining Solution
10X Washing Solution
2X Solubilization Solution
20 mM Doxorubicin
Gel Pack
Shelf Life
24 months from the date of purchase, if it is properly stored.
Research Use
For Research Use Only! Not For Use in Humans.

Figure: (a) The 72-hour dose-response curve of HepG2 (a) and MCF-7 (b) cells to adriamycin, as determined by the neutrophil cytotoxicity test.

All products and services are for Research Use Only. Do Not use in humans.


Creative Biolabs has established a team of customer support scientists ready to discuss ADCC/CDC optimization strategies, antibody production, bioinformatics analysis and other molecular biology/biotechnology issues.

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