ADP/ATP Ratio Assay Kit (CAT#: CB-P228-K) Datasheet

Kit

The change in the ADP/ATP ratio has been used to distinguish patterns of cell death and survival. Increased ATP levels and decreased ADP levels indicate proliferating cells. On the contrary, decreased ATP levels and increased ADP levels indicate apoptosis or necrosis of cells, necrosis Compared with apoptosis, the decrease of ATP and the increase of ADP are more obvious.

Creative Biolabs' ADP/ATP ratio analysis kit provides a quick method to measure ADP and ATP levels to screen for apoptosis, necrosis and cell proliferation in mammalian cells. The measurement involves two steps. In the first step, the working reagent lyses the cells to release ATP and ADP. In the presence of luciferase, ATP immediately reacts with the substrate D-luciferin to produce light. Light intensity is a direct measure of the ATP concentration in the cell. In the second step, ADP is converted to ATP through an enzymatic reaction. Then like the first step, this newly formed ATP reacts with D-luciferin. This non-radioactive, homogeneous cell-based assay is performed in microplates. This reagent is compatible with all media and all liquid handling systems, and can be used for high-throughput screening applications in 96-well and 384-well plates.