Product Type
Antibody
Description
The orphan receptor C5L2/pgpr77, which has 35% amino acid identity with CD88, is a promiscuous complement fragment binding protein. C5L2 binds to C5a with high affinity, but its affinity for C5adR is 10 times higher than that of CD88. C5L2 also has affinity for C4a and C3a and their metabolites C4a des-Arg and C3a des-Arg. C3a binds at a site different from the C5a binding site. C5L2 transfected into cells does not support degranulation or increase in intracellular [Ca2 +], and does not rapidly internalize due to ligand binding. However, the connection of anaphylactoxin to C5L2 can enhance the degranulation response to high-affinity IgE receptor cross-linking, which indicates that C5L2 is an anaphylactoxin binding protein with unique ligand binding and signaling properties. It is recommended to use C5L2 to mediate the acylation stimulating properties of C3a des-Arg/ASP. C5L2 is expressed in granulocytes, mature dendritic cells, adipose tissue and fibroblasts.
Disease
Pulmonology
Size
100 µg
Species
Human
Clonality
Polyclonal
Buffer
PBS, containing 0.02% sodium azide and 0.1% bovine serum albumin.
Application
Flow cytometry, Frozen sections, Immuno fluorescence, Western blot
Application Notes
For flow cytometry, immunohistology, western blotting and immunofluorescence dilutions, it depends on the detection system used. It is recommended that users test reagents and determine their own optimal dilution. The typical initial working dilution is 1:50.
Storage Instructions
Product should be stored at 4°C. Under recommended storage conditions, product is stable for at least one year.
Alternative Names
C5AR2; C5L2; GPF77; GPR77; complement component 5a receptor 2
