CreaClean™ T1 Glycan Clean-up Cartridges [For Glycan Clean Up] (CAT#: CB-P278-K) Datasheet

Product Type
Kit
Description
The CreaClean™ T1 filter cartridge is designed to purify fluorophore-labeled glycans. The filter cartridges are designed for vacuum manifolds or liquid handling robots. They can also be used as stand-alone devices, and users can rely on gravity for cleaning and elution step.
Used to purify CreaTag™ fluorophore and chromophore-labeled glycans after labeling. Suitable for cleaning after labeling glycans with 2-AB (2-aminobenzamide) and 2-AA (2-aminobenzoic acid).
Features
Binding Capacity: Each cartridge can typically bind glycans released from up to 50-100 µg glycoprotein.
For higher capacity cleanup use LC-A-Ax cartridges.
Number of Samples: CreaClean™ T1 cartridges are designed for single use only.
Suitable Samples: A wide range of glycans can be purified. These include N-linked and O-linked type
oligosaccharides.
Binding Selectivity: Essentially stoichiometric binding and elution for most complex glycan mixtures.
Applications
The cartridges contain a unique solid phase extraction (SPE) resin that binds a wide range of fluorescently labeled glycans and allows purification of these from labeling reagents. They are compatible with many leading vacuum manifold SPE handling systems. Labelled glycans can be N or O-link type.
Sample
Glycoproteins and glycopeptides containing N-linked glycans
Requires
• Pure water (HPLC grade)
• Methanol
• 1M sodium hydroxide (aq)
• 30% acetic acid (aq)
• Acetonitrile (HPLC grade)
• Trifluoroacetic acid (Analar grade)
• Wash A: 5 % (v/v) acetonitrile (aq) plus 0.1% trifluoroacetic acid
• Wash B: 50 % (v/v) acetonitrile (aq) plus 0.1% trifluoroacetic acid
• Pipettes
• Microcentrifuge
Handling Advice
Ensure that any glass, plasticware or solvents used are free of glycosidases and environmental carbohydrates. Use powder-free gloves for all sample handling procedures and avoid contamination with environmental carbohydrate.
Shipping
The product should be shipped at ambient temperature.
Storage
Store at room temperature in the dark. Protect from sources of heat, light, and moisture. The cartridges are stable for at least two years as supplied.
Shelf Life
Stability: Stable at least 12 months when stored properly. Several days exposure to ambient tempertures will not reduce activity.
Research Use
For research use only. Not for human or drug use

All products and services are for Research Use Only. Do Not use in humans.

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