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Sialidase Cp α(2-3,6) [For Glycan Release] (CAT#: CB-P263-K) Datasheet

Product Type
Kit
Description
Α(2-3,6) sialidase Cp cleaves all non-reducing terminal and non-branched α(2-3) and α(2-6) sialic acid residues in complex carbohydrates and glycoproteins. For α (2-8) or α(2-9) linkage or branched α(2-3) or α(2-6) linkage. The relative cleavage rate of different linkages is: α(2-3)>α(2- 6).
Α(2-3,6) sialidase Cp was isolated from the clone of Clostridium perfringens. The enzyme has been extensively characterized using oligosaccharide standards.
Features
Source: Recombinant from Clostridium perfringens in E. coli.

EC: 3.2.1.18

Alternate Names: Neuraminidase, NANase, N-acetylneuraminate glycohydrolase, Exo-α-sialidase

Specific Activity:>250 U/mg
Activity:>15 U/mL

Molecular weight: 41,000 daltons

pH range: 50 mM sodium phosphate (pH 6.0) provides the optimal buffer for enzyme activity with 3'-siayllactose, a standard substrate. If glycosidase treatment is performed at suboptimal pH because of glycoprotein solubility or activity requirements, expect some diminution in enzyme activity.

Specificity: Cleaves all nonreducing terminal non-branched α(2-3) and α(2-6) sialic acid residues from complex carbohydrates and glycoproteins.

Specific Activity Assay: Defined as the amount of enzyme required to produce 1 µmole of methylumbelliferone in 1 minute at 37°C, pH 5.0 from MU-NANA [2'-(4-methylumbelliferyl)-α-D-N-acetylneuraminic acid].
Applications
Sialidase Cp cleaves all non-reducing terminal non-branched α(2-3) and α(2-6) sialic acid residues from complex carbohydrates and glycoproteins.
Size
0.9 U / 60 µL; Sufficient for up to 60 reactions.
Sample
Glycoproteins and glycopeptides containing N-linked glycans
Components
Sialidase Cp in 20 mM Tris-HCl, 25 mM NaCl, pH 7.5
5x Reaction Buffer 250 mM sodium phosphate, pH 6.0
Handling Advice
1. Add up to 100 µg of glycoprotein or 1 nmol of oligosaccharide to tube.
2. Add water to 14 µL
3. Add 4 µL 5X Reaction Buffer.
4. Add 2 µL α(2-3,6) Sialidase Cp.
5. Incubate at 37°C for 1 hour.
Storage
Storage: Store enzyme at 4°C.
Shelf Life
Stability: Stable at least 12 months when stored properly. Several days exposure to ambient tempertures will not reduce activity.
Research Use
For research use only. Not for human or drug use

All products and services are for Research Use Only. Do Not use in humans.

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