Glucose-1-Phosphate Assay Kit (Colorimetric) (CAT#: CB-P169-K) Datasheet

Product Type
Kit
Description
In the glucose-1-phosphate assay kit, in the presence of glucose 1,6-diphosphate glucose, phospho-phosphate mutase converts G1P (glucose-1-phosphate) into glucose 6-phosphate; subsequently, glucose- Glucose 6-phosphate is oxidized by glucose-6-phosphate dehydrogenase to form NADH, which reduces the colorless probe to a colored product with strong absorption at 450 nm. Creative Biolabs's Glucose 1-Phosphate Assay Kit is fast, sensitive and easy to use. It can detect 1µM-10 mM G1P. The assay kit can be used for a variety of sample types.
Applications
Functional Studies
Size
1 kit
Detection Method
Colorimetric/Fluorometric
Detection Limit
1 µM - 10000 µM
Sensitivity
= 1 µM
Sample
Serum, Plasma, Other biological fluids, Tissue, Adherent cells, Suspension cells
Species Reactivity
Mammals
Protocol Length
0h 40m
Components
G1P Assay Buffer 1 x 27ml
G1P Developer 1 vial
G1P Enzyme Mix 1 vial
G1P Standard 1 vial
G1P Substrate Mix 1 vial
Requires
Microplate reader
Storage
Please refer to protocols.
Shelf Life
12 months
Research Use
For research use only. Not for human or drug use

Figure 1 Measurement of glucose-1-phosphate in jackfruit seed, bovine liver & jurkat cell lysate.

Figure 2 Typical Standard Curve

All products and services are for Research Use Only. Do Not use in humans.

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