Book a Meeting

Phosphoglucose Isomerase Activity Assay Kit (Colorimetric) (CAT#: CB-P175-K) Datasheet

Product Type
Kit
Description
Creative Biolabs' phosphoglucose isomerase activity assay kit (colorimetric) is a assay in which PGI (phosphoglucose isomerase) converts fructose 6 phosphate into glucose 6 phosphate. Glucose-6-phosphate is oxidized by glucose-6-phosphate dehydrogenase to form a product, which reacts with a colorless probe to produce a strong absorption at 450 nm. PGI assay is simple, sensitive and fast, and can detect glucose phosphate isomerase activity of less than 0.1 mU/reaction.
Applications
Functional Studies
Size
1 kit
Detection Method
Colorimetric/Fluorometric
Detection Limit
2.5 nmol/well - 12.5 nmol/well
Sensitivity
< 0.1 mU/well
Sample
Tissue, Adherent cells, Suspension cells, Tissue Homogenate
Species Reactivity
Mammals
Protocol Length
0h 40m
Components
NADH Standard 1 vial
PGI Assay Buffer 1 x 27ml
PGI Developer 1 vial
PGI Enzyme Mix 1 vial
PGI Positive Control 1 vial
PGI Substrate 1 vial
Requires
Microplate reader
Storage
Please refer to protocols.
Shelf Life
12 months
Research Use
For research use only. Not for human or drug use

Figure 1 NADH standard curve generated useing the method described in the protocol booklet.

Figure 2 Phosphoglucose Isomerase activity in bovine liver, HeLa cell lysate and positive control

Related Products

Malate Assay Kit (Colorimetric) (CAT#: CB-P187-K)
NGA2 Glycan (A2, G0) [N-Glycan Standards] (CAT#: CB-P312-K)
NGA2F Glycan (FA2, G0F), APTS labeled [Labeled Glycan Standards] (CAT#: CB-P391-K)
Antibody IgG N-glycosylation kit, Neutral (CAT#: CB-P192-K)
A1F Glycan (FA2G2S1, G2FS1), Procainamide labelled [Labeled Glycan Standards] (CAT#: CB-P414-K)
α(1-2,3,6)-mannosidase [For Glycan Release] (CAT#: CB-P269-K)
NA2F Glycan (FA2G2, G2F), 2-AB Labelled [Labeled Glycan Standards] (CAT#: CB-P331-K)
Galactose Assay Kit (Colorimetric/Fluorometric) (CAT#: CB-P176-K)

All products and services are for Research Use Only. Do Not use in humans.

ONLINE INQUIRY

Creative Biolabs has established a team of customer support scientists ready to discuss ADCC/CDC optimization strategies, antibody production, bioinformatics analysis and other molecular biology/biotechnology issues.

  • *
  • *
  • *
USA

UK

Germany