Phosphoglucose Isomerase Activity Assay Kit (Colorimetric) (CAT#: CB-P175-K) Datasheet

Product Type
Kit
Description
Creative Biolabs' phosphoglucose isomerase activity assay kit (colorimetric) is a assay in which PGI (phosphoglucose isomerase) converts fructose 6 phosphate into glucose 6 phosphate. Glucose-6-phosphate is oxidized by glucose-6-phosphate dehydrogenase to form a product, which reacts with a colorless probe to produce a strong absorption at 450 nm. PGI assay is simple, sensitive and fast, and can detect glucose phosphate isomerase activity of less than 0.1 mU/reaction.
Applications
Functional Studies
Size
1 kit
Detection Method
Colorimetric/Fluorometric
Detection Limit
2.5 nmol/well - 12.5 nmol/well
Sensitivity
< 0.1 mU/well
Sample
Tissue, Adherent cells, Suspension cells, Tissue Homogenate
Species Reactivity
Mammals
Protocol Length
0h 40m
Components
NADH Standard 1 vial
PGI Assay Buffer 1 x 27ml
PGI Developer 1 vial
PGI Enzyme Mix 1 vial
PGI Positive Control 1 vial
PGI Substrate 1 vial
Requires
Microplate reader
Storage
Please refer to protocols.
Shelf Life
12 months
Research Use
For research use only. Not for human or drug use

Figure 1 NADH standard curve generated useing the method described in the protocol booklet.

Figure 2 Phosphoglucose Isomerase activity in bovine liver, HeLa cell lysate and positive control

All products and services are for Research Use Only. Do Not use in humans.

ONLINE INQUIRY

Creative Biolabs has established a team of customer support scientists ready to discuss ADCC/CDC optimization strategies, antibody production, bioinformatics analysis and other molecular biology/biotechnology issues.

  • *
  • *
  • *
USA

UK