Product Type
Kit
Description
Creative Biolabs' phosphoglucose isomerase activity assay kit (colorimetric) is a assay in which PGI (phosphoglucose isomerase) converts fructose 6 phosphate into glucose 6 phosphate. Glucose-6-phosphate is oxidized by glucose-6-phosphate dehydrogenase to form a product, which reacts with a colorless probe to produce a strong absorption at 450 nm. PGI assay is simple, sensitive and fast, and can detect glucose phosphate isomerase activity of less than 0.1 mU/reaction.
Applications
Functional Studies
Size
1 kit
Detection Method
Colorimetric/Fluorometric
Detection Limit
2.5 nmol/well - 12.5 nmol/well
Sensitivity
< 0.1 mU/well
Sample
Tissue, Adherent cells, Suspension cells, Tissue Homogenate
Protocol Length
0h 40m
Components
NADH Standard 1 vial
PGI Assay Buffer 1 x 27ml
PGI Developer 1 vial
PGI Enzyme Mix 1 vial
PGI Positive Control 1 vial
PGI Substrate 1 vial
Requires
Microplate reader
Storage
Please refer to protocols.
Shelf Life
12 months
Research Use
For research use only. Not for human or drug use
