Product Type
Antibody
Description
The monoclonal antibody 20/70 recognizes the mouse receptor of C5a (CD88). Mouse anaphylactoxin complement factor C5a is a split product of C5, one of the most effective inflammatory chemokines, and interacts with two C51 receptors, C5aR and C5L2. Most C5a effects are mediated through C5aR. It is known that C5aR is essential in triggering acute inflammation. C5a receptors are seven transmembrane GTP-binding protein-coupled receptors, belonging to the rhodopsin supergene family. The 45 kDa C5a receptor is expressed by white blood cells, such as neutrophils, eosinophils, basophils, monocytes, dendritic cells, and mast cells. C5aR can be expressed on immune and non-immune cells. Non-immune cells expressing C5aR include vascular endothelial cells, cardiomyocytes and bronchial epithelial cells. C5aR signaling involves intracellular calcium mobilization and activation of protein kinase B (PKB) and protein phospholipase D (PLD) pathways. High C5aR expression levels have been associated with the pathogenesis of many inflammatory diseases, autoimmune and neurodegenerative diseases such as rheumatoid arthritis, multiple sclerosis, atherosclerosis and inflammatory bowel disease. The blockade of C5aR in the body greatly reduces inflammatory damage. The monoclonal antibody 20/70 has been used in functional studies and can prevent C5a from binding to its receptor.
Disease
Infectious diseases
Size
100 µg
Immunogen
RBL-2H3 transfectants expressing murine C5aR on the cell surface.
Species
Mouse
Clonality
Monoclonal
Clone Number
20/70
Buffer
PBS, containing 0.1% bovine serum albumin.
Application
Flow cytometry, Functional studies, Immuno fluorescence
Application Notes
The diluent used depends on the detection system used. It is recommended that users test reagents and determine their own optimal dilution. The typical initial working dilution is 1:50. For functional studies, the in vitro dilution must be optimized in the user's experimental setting.
Storage Instructions
Product should be stored at 4°C. Under recommended storage conditions, product is stable for at least one year.
Alternative Names
C5aR1; CD88; C5aR; CD88; C5a anaphylatoxin chemotactic receptor; C5a anaphylatoxin receptor; C5a ligand; C5a-R; complement component 5a receptor 1
