Product Type
Antibody
Description
The monoclonal antibody 10/92 recognizes the mouse receptor C5a (C5aR, CD88). Mouse anaphylactoxin C5a is a 74-residue glycopolypeptide, which is produced by proteolytic cleavage of complement factor C5 during complement activation. It can be proved that the various biological effects caused by C5a make it take granulocytes and macrophages as the main target cells and become important mediators of inflammation. All cellular responses to C5a are mediated through interaction with membrane-bound C5a receptors, which are seven transmembrane GTP-binding protein-coupled receptors of the rhodopsin supergene family. The 45 kDa C5a receptor is expressed by neutrophils, monocytes/macrophages, dendritic cells, astrocytes and microglia. In contrast, C5a receptors were not detected on resting or stimulated murine T and B lymphocytes. The monoclonal antibody 10/92 does not inhibit the binding of C5a to its receptor.
Disease
Infectious diseases
Size
100 µg
Immunogen
RBL-2H3 transfectants expressing murine C5aR
Species
Mouse
Clonality
Monoclonal
Clone Number
10/92
Buffer
PBS, containing 0.1% bovine serum albumin and 0.02% sodium azide.
Application
Flow cytometry, Frozen sections, Western blot
Application Notes
For immunohistology, Western blotting and flow cytometry, the diluent to be used depends on the detection system used. It is recommended that users test reagents and determine their own optimal dilution. The typical initial working dilution is 1:50.
Storage Instructions
Product should be stored at 4°C. Under recommended storage conditions, product is stable for at least one year.
Alternative Names
C5aR1; CD88; C5aR; CD88; C5a anaphylatoxin chemotactic receptor; C5a anaphylatoxin receptor; C5a ligand; C5a-R; complement component 5a receptor 1
