Mouse Fc ELISA Complete Kit (CAT#: CB-P223-K) Datasheet

Kit

Fc is the tail region of immunoglobulin G (IgG), which interacts with cell surface receptors called Fc receptors and certain proteins of the complement system. Fragments of ~230 amino acids usually exist as dimers, Although under reducing conditions, it is the basis for prolonging antibody pharmacokinetics, and is usually used as a fusion to extend the half-life of the fusion protein.

The assay uses quantitative sandwich enzyme immunoassay technology. Affinity purified polyclonal antibodies specific to mouse or mouse Fc have been coated on 96-well microtiter plates. Pipette the standard solution, control solution and sample into the wells, and any Mouse or Mouse Fc present will be bound by the immobilized antibody. After washing away all unbound material, an enzyme-linked polyclonal antibody specific for mouse or mouse Fc is added to the well. After incubating and washing to remove any unbound antibody-enzyme reagent, the substrate solution is added to the well. The enzyme reaction produces a blue product, which turns yellow when the stop solution is added. The measured color intensity is proportional to the amount of Mouse or Mouse Fc bound in the initial step. Then read the sample value from the standard curve.

The Mouse Fc ELISA Complete kit contains the same basic components as the Mouse Fc ELISA kit (CB-P223-K).