LDH Assay Kit (Cytotoxicity) (CAT#: CB-P181-K) Datasheet

Product Type
Kit
Description
For a more sensitive LDH test kit for cytotoxicity testing, we recommend using CB-P150-K. The LDH test kit (cytotoxicity) is based on the measurement of lactate dehydrogenase (LDH) activity released by damaged cells, Provides a quick and easy way to quantify cytotoxicity
The measurement requires about 0.5-1 hour. The activity of LDH can be determined by a coupled enzymatic reaction: LDH oxidizes lactic acid to pyruvate and then reacts with the tetrazolium salt INT to form formazan. The increase in the number of formazan maz produced in the culture supernatant is directly related to the increase in the number of lysed cells. Formaldehyde dye is water-soluble and can be detected at 500 nm with a spectrophotometer.
Applications
Functional Studies
Size
1 kit
Detection Method
Colorimetric/Fluorometric
Sample
Adherent cells, Suspension cells
Species Reactivity
Mammals
Protocol Length
2h 00m
Components
Catalyst (Lyophilized) 1 vial
Dye Solution 1 x 45ml
Requires
Microplate reader
Storage
Please refer to protocols.
Shelf Life
12 months
Research Use
For research use only. Not for human or drug use

Figure 1 LDH release measured at various cell amounts.

Figure 2 Jurkat cells were cultured in a 96-well plate in 100 µl of culture medium. LDH assay was performed using 10 µl of culture medium according to the kit instructions. Light bar: Low control; Dark bar: High control.

All products and services are for Research Use Only. Do Not use in humans.

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