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Maltose and Glucose Assay Kit (Colorimetric/Fluorometric) (CAT#: CB-P190-K) Datasheet

Product Type
Kit
Description
Maltose and glucose assay kits can measure glucose or maltose levels in various biological samples (such as serum, plasma, body fluids, food, growth medium, etc.). The assay is based on the ability of glucose oxidase to specifically oxidize free glucose to produce products that interact with glucose probes. The amount of the product can be colorimetric (OD = 570 nm spectrophotometry) or Fluorometric (Ex / Em = 535/587 nm).
Applications
Functional Studies
Size
1 kit
Detection Method
Colorimetric/Fluorometric
Detection Limit
0.01 nmol/well - 10 nmol/well
Sensitivity
> 0.01 nmol/well
Sample
Cell culture supernatant, Urine, Serum, Plasma, Tissue, Adherent cells, Suspension cells, Tissue Homogenate
Species Reactivity
Mammals
Protocol Length
1h 00m
Components
alpha-D-Glucosidase (Lyophilized) 1 vial
Assay Buffer 1 x 25ml
Glucose Enzyme Mix (lyophilized) 1 vial
Glucose Probe 1 x 0.2ml
Maltose Standard (100 nmol/µl) 1 x 100µl
Requires
Microplate reader
Storage
Please refer to protocols.
Shelf Life
12 months
Research Use
For research use only. Not for human or drug use

Figure 1 Maltose Standard Curve. Assays were performed using various amounts of Maltose standard according to kit instructions. Circle is maltose with a-D-Glucosidase. Square is maltose without a-D-Glucosidase. Triangle is glucose with 2 times nmol of Maltose.

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All products and services are for Research Use Only. Do Not use in humans.

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