ALDH Activity Assay Kit (Colorimetric) (CAT#: CB-P147-K) Datasheet

Product Type
Kit
Description
ALDH activity assay kit (colorimetric) is a simple, rapid and reliable to quantify ALDH enzyme activity. In this assay, acetaldehyde is oxidized by ALDH to generate NADH, and then the colorless probe is reduced to a colored product with a strong absorbance at 450 nm. This assay can detect ALDH activity <0.5 mU in various samples.
Applications
Functional Studies
Size
1 kit
Detection Method
Colorimetric
Sensitivity
< 0.5 mU/well
Sample
Tissue, Adherent cells, Suspension cells
Species Reactivity
Mammals
Protocol Length
0h 30m
Components
Acetaldehyde 1 x 0.5ml
ALDH Assay Buffer 1 x 25ml
ALDH Positive Control 1 vial
ALDH Substrate Mix 1 vial
NADH Standard 1 vial
Requires
Microplate reader
Handling Advice
ALDH assay protocol summary:
- add samples and standards to wells
- add reaction mix and incubate for 20-60 min
- analyze with a microplate reader at 5 min and 20-60 min
Storage
Please refer to protocols.
Shelf Life
12 months
Research Use
For research use only. Not for human or drug use

Figure 1 Standard curve (60 minutes incubation) showing mean of duplicates (+/- SD) with background reads subtracted.


Figure 2 Typical OD of the diffrent positive contols mesuared over 1 hour.


Figure 3 ALDH activity measured in human biological fluids (T1=5 min; T2=60 min), showing activity (mU) per mL of tested sample, background signal subtracted.

All products and services are for Research Use Only. Do Not use in humans.

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