Connect with Us at the Upcoming Events

Creative Biolabs is excited to greet you at the upcoming international conferences. Meet our team at the 13th Annual World ADC San Diego and the 13th Annual World Multispecific Summit in September (booth number to be updated) for expert consultation on your drug discovery. Shoot an email to arrange an in-person meeting!

Book a Meeting

ALDH Activity Assay Kit (Colorimetric) (CAT#: CB-P147-K) Datasheet

Product Type
ALDH activity assay kit (colorimetric) is a simple, rapid and reliable to quantify ALDH enzyme activity. In this assay, acetaldehyde is oxidized by ALDH to generate NADH, and then the colorless probe is reduced to a colored product with a strong absorbance at 450 nm. This assay can detect ALDH activity <0.5 mU in various samples.
Functional Studies
1 kit
Detection Method
< 0.5 mU/well
Tissue, Adherent cells, Suspension cells
Species Reactivity
Protocol Length
0h 30m
Acetaldehyde 1 x 0.5ml
ALDH Assay Buffer 1 x 25ml
ALDH Positive Control 1 vial
ALDH Substrate Mix 1 vial
NADH Standard 1 vial
Microplate reader
Handling Advice
ALDH assay protocol summary:
- add samples and standards to wells
- add reaction mix and incubate for 20-60 min
- analyze with a microplate reader at 5 min and 20-60 min
Please refer to protocols.
Shelf Life
12 months
Research Use
For research use only. Not for human or drug use

Figure 1 Standard curve (60 minutes incubation) showing mean of duplicates (+/- SD) with background reads subtracted.

Figure 2 Typical OD of the diffrent positive contols mesuared over 1 hour.

Figure 3 ALDH activity measured in human biological fluids (T1=5 min; T2=60 min), showing activity (mU) per mL of tested sample, background signal subtracted.

All products and services are for Research Use Only. Do Not use in humans.


Creative Biolabs has established a team of customer support scientists ready to discuss ADCC/CDC optimization strategies, antibody production, bioinformatics analysis and other molecular biology/biotechnology issues.

  • *
  • *
  • *