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ALDH Activity Assay Kit (Colorimetric) (CAT#: CB-P147-K) Datasheet

Product Type
Kit
Description
ALDH activity assay kit (colorimetric) is a simple, rapid and reliable to quantify ALDH enzyme activity. In this assay, acetaldehyde is oxidized by ALDH to generate NADH, and then the colorless probe is reduced to a colored product with a strong absorbance at 450 nm. This assay can detect ALDH activity <0.5 mU in various samples.
Applications
Functional Studies
Size
1 kit
Detection Method
Colorimetric
Sensitivity
< 0.5 mU/well
Sample
Tissue, Adherent cells, Suspension cells
Species Reactivity
Mammals
Protocol Length
0h 30m
Components
Acetaldehyde 1 x 0.5ml
ALDH Assay Buffer 1 x 25ml
ALDH Positive Control 1 vial
ALDH Substrate Mix 1 vial
NADH Standard 1 vial
Requires
Microplate reader
Handling Advice
ALDH assay protocol summary:
- add samples and standards to wells
- add reaction mix and incubate for 20-60 min
- analyze with a microplate reader at 5 min and 20-60 min
Storage
Please refer to protocols.
Shelf Life
12 months
Research Use
For research use only. Not for human or drug use

All products and services are for Research Use Only. Do Not use in humans.

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