Sialidase Sp α(2-3) [For Glycan Release] (CAT#: CB-P258-K) Datasheet

Kit

Sialidase Sp cleaves the non-reducing end α(2-3) unbranched sialic acid residues from complex carbohydrates and glycoproteins.
α(2-3) Sialidase Sp only cleaves the non-reducing terminal α(2-3) branched sialic acid residues from complex carbohydrates and glycoproteins. There is no detectable activity on α(2-6) or α(2-8) linkage or branched α(2-3) linkage. To cleave all non-reducing terminal sialic acid residues from complex carbohydrates and glycoproteins, including branched sialic acid (connected to internal residues), use α(2-3,6,8,9) sialidase Au (catalog number CB-P257-K)).

Source: Recombinant Streptococcus pneumoniae in E. coli

EC: 3.2.1.18

Alternate Names: Neuraminidase, N-acetylneuraminate glycohydrolase, Exo-α-sialidase

Specific Activity: = 250 U/mg
Activity: = 10 U/mL

Molecular weight: ~75,000 daltons

pH optimum 6.0

Specificity: Cleaves the non-reducing terminal α(2-3) unbranched sialic acid residues from complex carbohydrates and glycoproteins.

Specific Activity: Defined as the amount of enzyme required to produce 1 µmole of methylumbelliferone in 1 minute at 37°C, pH 5.0 from MU-NANA [2'-(4-methylumbelliferyl)-α-D-N-acetylneuraminic acid].