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Lactate Dehydrogenase (LDH) Assay Kit (Fluorometric) (CAT#: CB-P157-K) Datasheet

Product Type
Kit
Description
The Lactate Dehydrogenase (LDH) Assay Kit (Fluorometric) provides a quick and easy to monitor lactate dehydrogenase (LDH) activity in a variety of samples. In this assay, LDH converts lactic acid into pyruvate and NADH, the latter reacts with a specific fluorescent probe to produce a strong fluorescent product (Ex / Em = 535/587 nm).
Applications
Functional Studies
Size
1 kit
Detection Method
Fluorometric
Sensitivity
1 uU/ml
Sample
Cell Lysate, Tissue Lysate
Species Reactivity
Mammals
Protocol Length
0h 30m
Components
LDH Assay Buffer 1 x 110ml
LDH Substrate Mix 1 vial
NADH Standard 1 vial
PicoProbe 1 x 1.4ml
Positive Control 1 vial
Requires
Microplate reader
Handling Advice
LDH assay protocol summary:
- add samples and standards to wells
- add reaction mix
- analyze with microplate reader every 2-3 min for 10-30 min
Storage
Please refer to protocols.
Shelf Life
12 months
Research Use
For research use only. Not for human or drug use

Figure 1 Functional Studies
Typical NADH Standard Curve obtained following assay protocol.


Figure 2 Functional Studies
Kinetic measurement of Lactate Dehydrogenase activity in a range of biological samples.


Figure 3 Functional Studies
Relative LDH Activity was calculated in lysates prepared from rat liver (0.037 µg protein), Jurkat cells (0.053 µg protein), and Human serum (0.2 µg protein).

All products and services are for Research Use Only. Do Not use in humans.

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