Product Type
Kit
Description
The Lactate Dehydrogenase (LDH) Assay Kit (Fluorometric) provides a quick and easy to monitor lactate dehydrogenase (LDH) activity in a variety of samples. In this assay, LDH converts lactic acid into pyruvate and NADH, the latter reacts with a specific fluorescent probe to produce a strong fluorescent product (Ex / Em = 535/587 nm).
Applications
Functional Studies
Size
1 kit
Detection Method
Fluorometric
Sensitivity
1 uU/ml
Sample
Cell Lysate, Tissue Lysate
Protocol Length
0h 30m
Components
LDH Assay Buffer 1 x 110ml
LDH Substrate Mix 1 vial
NADH Standard 1 vial
PicoProbe 1 x 1.4ml
Positive Control 1 vial
Requires
Microplate reader
Handling Advice
LDH assay protocol summary:
- add samples and standards to wells
- add reaction mix
- analyze with microplate reader every 2-3 min for 10-30 min
Storage
Please refer to protocols.
Shelf Life
12 months
Research Use
For research use only. Not for human or drug use
