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Lactate Dehydrogenase (LDH) Assay Kit (Fluorometric) (CAT#: CB-P157-K) Datasheet

Product Type
The Lactate Dehydrogenase (LDH) Assay Kit (Fluorometric) provides a quick and easy to monitor lactate dehydrogenase (LDH) activity in a variety of samples. In this assay, LDH converts lactic acid into pyruvate and NADH, the latter reacts with a specific fluorescent probe to produce a strong fluorescent product (Ex / Em = 535/587 nm).
Functional Studies
1 kit
Detection Method
1 uU/ml
Cell Lysate, Tissue Lysate
Species Reactivity
Protocol Length
0h 30m
LDH Assay Buffer 1 x 110ml
LDH Substrate Mix 1 vial
NADH Standard 1 vial
PicoProbe 1 x 1.4ml
Positive Control 1 vial
Microplate reader
Handling Advice
LDH assay protocol summary:
- add samples and standards to wells
- add reaction mix
- analyze with microplate reader every 2-3 min for 10-30 min
Please refer to protocols.
Shelf Life
12 months
Research Use
For research use only. Not for human or drug use

Figure 1 Functional Studies
Typical NADH Standard Curve obtained following assay protocol.

Figure 2 Functional Studies
Kinetic measurement of Lactate Dehydrogenase activity in a range of biological samples.

Figure 3 Functional Studies
Relative LDH Activity was calculated in lysates prepared from rat liver (0.037 µg protein), Jurkat cells (0.053 µg protein), and Human serum (0.2 µg protein).

All products and services are for Research Use Only. Do Not use in humans.


Creative Biolabs has established a team of customer support scientists ready to discuss ADCC/CDC optimization strategies, antibody production, bioinformatics analysis and other molecular biology/biotechnology issues.

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