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Hexokinase Activity Assay Kit (Colorimetric) (CAT#: CB-P143-K) Datasheet

Product Type
The hexokinase activity assay kit is simple to use, sensitive, and fast. It can detect hexokinase activity below 0.1 mU/well.
In the hexokinase assay protocol, hexokinase converts glucose into glucose 6-phosphate. Glucose 6-phosphate is then oxidized by glucose 6-phosphate dehydrogenase to form NADH. NADH reduces the colorless probe to a colored product with strong absorbance at 450 nm.
Functional Studies
1 kit
Detection Method
< 0.1 mU/well
Serum, Tissue, Adherent cells, Suspension cells, Tissue Extracts
Species Reactivity
Protocol Length
1h 00m
Assay Buffer 1 x 25ml
Developer (Lyophilized) 1 vial
Hexokinase Coenzyme 1 vial
Hexokinase Enzyme Mix 1 vial
Hexokinase Substrate 1 x 1ml
NADH Standard (lyophilized) 1 vial
Positive Control 1 vial
Microplate reader
Handling Advice
Hexokinase activity assay protocol summary:
- add samples and standards to wells
- add reaction mix and incubate for 20-60 min
- analyze repeatedly with a microplate reader during the incubation
Please refer to protocols.
Shelf Life
12 months
Research Use
For research use only. Not for human or drug use

Figure 1 Hexokinase Activity measured in cell lysates showing quantity (nmol) per 1 mln cells after 35 min of incubation.

Figure 2 Hexokinase Activity measured in tissue lysates showing quantity (nmol) per mg of extracted protein after 35 min of incubation.

Figure 3 Standard curve: mean of duplicates (+/- SD) with background reads subtracted

Figure 4 NADH standard curve (a). Hexokinase activity in Positive Control and Jurkat cell lysate (40 µg) (b).

All products and services are for Research Use Only. Do Not use in humans.


Creative Biolabs has established a team of customer support scientists ready to discuss ADCC/CDC optimization strategies, antibody production, bioinformatics analysis and other molecular biology/biotechnology issues.

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