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Hexokinase Activity Assay Kit (Colorimetric) (CAT#: CB-P143-K) Datasheet

Product Type
Kit
Description
The hexokinase activity assay kit is simple to use, sensitive, and fast. It can detect hexokinase activity below 0.1 mU/well.
In the hexokinase assay protocol, hexokinase converts glucose into glucose 6-phosphate. Glucose 6-phosphate is then oxidized by glucose 6-phosphate dehydrogenase to form NADH. NADH reduces the colorless probe to a colored product with strong absorbance at 450 nm.
Applications
Functional Studies
Size
1 kit
Detection Method
Colorimetric/Fluorometric
Sensitivity
< 0.1 mU/well
Sample
Serum, Tissue, Adherent cells, Suspension cells, Tissue Extracts
Species Reactivity
Mammals
Protocol Length
1h 00m
Components
Assay Buffer 1 x 25ml
Developer (Lyophilized) 1 vial
Hexokinase Coenzyme 1 vial
Hexokinase Enzyme Mix 1 vial
Hexokinase Substrate 1 x 1ml
NADH Standard (lyophilized) 1 vial
Positive Control 1 vial
Requires
Microplate reader
Handling Advice
Hexokinase activity assay protocol summary:
- add samples and standards to wells
- add reaction mix and incubate for 20-60 min
- analyze repeatedly with a microplate reader during the incubation
Storage
Please refer to protocols.
Shelf Life
12 months
Research Use
For research use only. Not for human or drug use

Figure 1 Hexokinase Activity measured in cell lysates showing quantity (nmol) per 1 mln cells after 35 min of incubation.


Figure 2 Hexokinase Activity measured in tissue lysates showing quantity (nmol) per mg of extracted protein after 35 min of incubation.


Figure 3 Standard curve: mean of duplicates (+/- SD) with background reads subtracted


Figure 4 NADH standard curve (a). Hexokinase activity in Positive Control and Jurkat cell lysate (40 µg) (b).

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All products and services are for Research Use Only. Do Not use in humans.

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