LDH Cytotoxicity Assay Kit (Fluorometric) (CAT#: CB-P152-K) Datasheet

Product Type
Kit
Description
The LDH assay kit (cytotoxicity, Fluorometric) provides a sensitive, fast, and simple to detect LDH (lactate dehydrogenase) released from damaged cells.

In LDH analysis, LDH converts lactic acid into pyruvate and NADH, thereby reducing the proprietary probe to a strong fluorescent product (Ex / Em = 535/587 nm).

The assay is suitable for high-throughput format and can be completed in less than 20 minutes. Sensitivity: ~100 cells. Each measurement requires 5µl of cell culture medium.
Applications
Functional Studies
Size
1 kit
Detection Method
Fluorometric
Sensitivity
100 cells/well
Sample
Adherent cells, Suspension cells
Species Reactivity
Mammals
Protocol Length
0h 20m
Components
Cell Lysis Solution 1 x 5ml
LDH Assay Buffer 1 x 50ml
LDH Substrate Mix 1 vial
PicoProbe 1 x 2ml
Positive Control 1 x 20µl
Requires
Microplate reader
Storage
Please refer to protocols.
Shelf Life
12 months
Research Use
For research use only. Not for human or drug use

Figure 1 Untreated Jurkat cells or treated with Cell Lysis Solution.


Figure 2 Relative Fluorescence Units of untreated Jurkat cells or treated with Cell Lysis Solution.
Jurkat T cells were cultured in 96-well plate in 100 μl of culture medium. LDH Assay was performed using 10μl of culture medium using the WST probe. Low control (white bar); High control (black bar).


Figure 3 Overnight treatment of HeLa cells with staurosporine or cycloheximide.
Comparison of WST-1 and INT based LDH assays. 3T3 cells were cultured in a 96-well plate in 100 µl of culture medium. The LDH assay was performed using 10 µl of culture medium using WST-1 (Brown bar) and INT (Green bar) methods. The WST-1 based LDH assay is more stable and sensitive than the INT based method.

All products and services are for Research Use Only. Do Not use in humans.

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