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LDH Cytotoxicity Assay Kit (Fluorometric) (CAT#: CB-P152-K) Datasheet

Product Type
Kit
Description
The LDH assay kit (cytotoxicity, Fluorometric) provides a sensitive, fast, and simple to detect LDH (lactate dehydrogenase) released from damaged cells.

In LDH analysis, LDH converts lactic acid into pyruvate and NADH, thereby reducing the proprietary probe to a strong fluorescent product (Ex / Em = 535/587 nm).

The assay is suitable for high-throughput format and can be completed in less than 20 minutes. Sensitivity: ~100 cells. Each measurement requires 5µl of cell culture medium.
Applications
Functional Studies
Size
1 kit
Detection Method
Fluorometric
Sensitivity
100 cells/well
Sample
Adherent cells, Suspension cells
Species Reactivity
Mammals
Protocol Length
0h 20m
Components
Cell Lysis Solution 1 x 5ml
LDH Assay Buffer 1 x 50ml
LDH Substrate Mix 1 vial
PicoProbe 1 x 2ml
Positive Control 1 x 20µl
Requires
Microplate reader
Storage
Please refer to protocols.
Shelf Life
12 months
Research Use
For research use only. Not for human or drug use

All products and services are for Research Use Only. Do Not use in humans.

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