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Hydrazinolysis Glycan Release Kit [For Glycan Release] (CAT#: CB-P272-K) Datasheet

Product Type
Kit
Description
The kit contains reagents for releasing N and O-linked glycans from glycoprotein biopharmaceuticals. The released glycans have free reducing ends and can be fluorescently labeled by reductive amination. The release conditions can be optimized for the release of N-glycans, O-glycans or both N- and O-glycans.
Features
Number of Samples: The kit contains reagents and materials for up to 12 glycoprotein samples analysed in parallel or two sets of 6.
Amount of Sample: Typically, up to 1 mg of glycoprotein per sample.
Suitable Samples: Biopharmaceutical glycoproteins.
Applications
For release of N + O-linked glycans from glycoprotein therapeutics
Sample
Glycoproteins and glycopeptides containing N-linked glycans
Components
Hydrazinolysis Release Reagent 2 x 3ml
Solid sodium bicarbonate 2 x 0.33 g
Octanol 2 x 0.2 ml
Acetic anhydride 2 x 0.5 ml
TFA solution 2 x 4 ml
CreaClean™ EB20 cartridges 2 x 6 cartridges
EB20 Wash A Solution 2 x 12 ml
EB20 Wash B Solution 2 x 12 ml
CreaClean™ CEX cartridges 2 x 6 cartridges
Wash waste vials 2 x 6 vials
Glass reaction vials with PTFE lined caps 2 x 6 vials
Glass collection vials with PTFE lined caps 2 x 6 vials
Handling Advice
1. Add up to 1 nmole of oligosaccharide to a tube.
2. Add de-ionized water to a total of 15 µL.
3. Add 4 µL of 5x Reaction Buffer 5.0.
4. Add 1 µL of α(1-3,4)-Fucosidase.
5. Incubate for 1 hour at 37°C.
Shipping
The product should be shipped at ambient temperature.
Storage
Store refrigerated at 4 - 10 oC in the dark.
Shelf Life
Stability: Stable at least 12 months when stored properly. Several days exposure to ambient tempertures will not reduce activity.
Research Use
For research use only. Not for human or drug use

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All products and services are for Research Use Only. Do Not use in humans.

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