Hydrazinolysis Glycan Release Kit [For Glycan Release] (CAT#: CB-P272-K) Datasheet

Product Type
Kit
Description
The kit contains reagents for releasing N and O-linked glycans from glycoprotein biopharmaceuticals. The released glycans have free reducing ends and can be fluorescently labeled by reductive amination. The release conditions can be optimized for the release of N-glycans, O-glycans or both N- and O-glycans.
Features
Number of Samples: The kit contains reagents and materials for up to 12 glycoprotein samples analysed in parallel or two sets of 6.
Amount of Sample: Typically, up to 1 mg of glycoprotein per sample.
Suitable Samples: Biopharmaceutical glycoproteins.
Applications
For release of N + O-linked glycans from glycoprotein therapeutics
Sample
Glycoproteins and glycopeptides containing N-linked glycans
Components
Hydrazinolysis Release Reagent 2 x 3ml
Solid sodium bicarbonate 2 x 0.33 g
Octanol 2 x 0.2 ml
Acetic anhydride 2 x 0.5 ml
TFA solution 2 x 4 ml
CreaClean™ EB20 cartridges 2 x 6 cartridges
EB20 Wash A Solution 2 x 12 ml
EB20 Wash B Solution 2 x 12 ml
CreaClean™ CEX cartridges 2 x 6 cartridges
Wash waste vials 2 x 6 vials
Glass reaction vials with PTFE lined caps 2 x 6 vials
Glass collection vials with PTFE lined caps 2 x 6 vials
Handling Advice
1. Add up to 1 nmole of oligosaccharide to a tube.
2. Add de-ionized water to a total of 15 µL.
3. Add 4 µL of 5x Reaction Buffer 5.0.
4. Add 1 µL of α(1-3,4)-Fucosidase.
5. Incubate for 1 hour at 37°C.
Shipping
The product should be shipped at ambient temperature.
Storage
Store refrigerated at 4 - 10 oC in the dark.
Shelf Life
Stability: Stable at least 12 months when stored properly. Several days exposure to ambient tempertures will not reduce activity.
Research Use
For research use only. Not for human or drug use

All products and services are for Research Use Only. Do Not use in humans.

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