Glucose Oxidase Activity Assay Kit (Colorimetric) (CAT#: CB-P165-K) Datasheet

Product Type
Kit
Description
The glucose oxidase activity assay kit uses a simple colorimetric assay to measure the glucose oxidase (GOx) activity in cell and tissue extracts and biological fluids. The assay can be performed in a convenient 96-well or 384-well format. And it is easy to adjust and can be automated without separation or cleaning steps.
The glucose oxidase assay protocol uses a red probe to monitor glucose oxidase activity, which leads to an increase in absorbance, which can be easily detected at OD575 nm.
Applications
Functional Studies
Size
1 kit
Detection Method
Colorimetric/Fluorometric
Sample
Cell Lysate
Species Reactivity
Mammals
Protocol Length
0h 20m
Components
Assay Buffer 1 x 1ml
DMSO 1 x 10ml
Glucose 1 vial
Glucose Oxidase (100 U) 1 vial
Horseradish Peroxidase (HRP) 1 x 17ml
Red Dye (light-sensitive) 1 x 1ml
Requires
Microplate reader
Handling Advice
Glucose oxidase assay protocol summary:
- add samples and standards to wells
- add glucose oxidase reaction mix
- analyze every 2-3 mins with a microplate reader in kinetic mode for at least 10-30 mins at 37°C
Storage
Please refer to protocols.
Shelf Life
12 months
Research Use
For research use only. Not for human or drug use

Figure 1 Glucose Oxidase Activity Assay.
Typical glucose oxidase dose response curve. Absorbance was measured on a 96-well clear bottom plate using a SpectraMax microplate reader with path check on. As low as 0.156 mU/mL glucose oxidase activity can be detected after 30 minutes incubation.

All products and services are for Research Use Only. Do Not use in humans.

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