Sialidase Testing Panel, Procainamide labelled [Labeled Glycan Standards] (CAT#: CB-P432-K) Datasheet

Product Type
Glycan Standards
Description
Sialidase Testing Panel is a glycan standard containing a mixture of α2-3, α2-6 and α2-8 sialylated oligosaccharides: 1. 3'-Sialyl Lewis X [Neu5Ac-a2-3Gal-b1-4(Fuc-a1-3)GlcNAc] - contains 2-3 linked sialic acid along with branched 1-3 fucose (introducing steric hindrance for some enzymatic reactions) 2. Sialyllacto-N-tetraose a [Neu5Ac-α2-3Gal-β1-3GlcNAc-β1-3Gal-β1-4Glc] - linear oligosaccharide containing 2-3 sialic acid attached to galactose 3. Disialyllactose [Neu5Ac-a2-8NeuAc-a2-3Gal-b1-4Glc] - linear oligosaccharide containing 2-8 linked sialic acid 4. Sialyllacto-N-tetraose c [Neu5Ac-a2-6Gal-b1-4GlcNAc-b1-3Gal-b1-4Glc] - linear oligosaccharide containing 2-6 sialic acid attached to galactose 5. Disialyllacto-N-tetraose [Neu5Ac-a2-3Gal-b1-3(Neu5Ac-a2-6)GlcNAc-b1-3Gal-b1- 4Glc] - branched sialic acid oligosaccharide
Features
Form Dry. Dried by centrifugal evaporation from an aqueous solution.
Size 100 pmol Molecular weight 1. 3'-Sialyl Lewis X 940.3648 2. Sialyllacto-N-tetraose a 1118.4126 3. Disialyllactose 1044.3758 4. Sialyllacto-N-tetraose c 1118.4126 5. Disialyllacto-N-tetraose 1409.5080 Purity 89% pure as assessed by UHPLC.
Sample
Glycoproteins and glycopeptides containing N-linked glycans
Handling Advice
Allow the unopened vial to reach ambient temperature and tap unopened on a solid surface to ensure that most of the lyophilized material is at the bottom of the vial. Gently remove the cap, add the desired volume of reconstitution medium, re-cap and mix thoroughly to bring all the oligosaccharide into solution. For maximal recovery of oligosaccharide, ensure that the cap lining is also rinsed and centrifuge the reconstituted vial briefly before use. Ensure that any glass, plasticware or solvents used are free of glycosidases and environmental carbohydrates.
Storage
Refridgerate (-20°C) both before and after dissolution. This product is stable for at least 5 years as supplied.
Shelf Life
Stability: Stable at least 12 months when stored properly. Several days exposure to ambient tempertures will not reduce activity.
Research Use
For research use only. Not for human or drug use

Figure 1 HILIC-UPLC profiles of procainamide labelled Sialidase Testing Panel

All products and services are for Research Use Only. Do Not use in humans.

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