Glycogen Assay Kit (Colorimetric/Fluorometric) (CAT#: CB-P149-K) Datasheet

Product Type
Kit
Description
The glycogen assay kit is a simple and accurate assay for measuring the glycogen content in biological samples. In the glycogen assay protocol, glucoamylase hydrolyzes glycogen into glucose, and then specifically oxidizes it to produce a product that reacts with the OxiRed probe to produce color (570 nm) and Fluorometric (Ex 535 / Em 587). This assay can detect glycogen at 0.0004 to 2mg/ml.
Applications
Functional Studies
Size
1 kit
Detection Method
Colorimetric/Fluorometric
Detection Limit
0.4 µg/ml - 2000 µg/ml
Sensitivity
> 0.4 µg/ml
Sample
Tissue, Adherent cells, Suspension cells
Species Reactivity
Mammals
Protocol Length
1h 00m
Components
Development Buffer 1 x 25ml
Development Enzyme Mix (lyophilized) 1 vial
Glycogen Standard (2.0 mg/ml) 1 x 100µl
Hydrolysis Buffer 1 x 25ml
Hydrolysis Enzyme Mix (lyophilized) 1 vial
OxiRed Probe 1 x 200µl
Requires
Microplate reader
Handling Advice
Glycogen assay protocol summary:
- add samples and standards to wells
- add hydrolysis enzyme mix and incubate for 30 min
- add reaction mix and incubate for 30 min
- analyze with microplate reader
Storage
Please refer to protocols.
Shelf Life
12 months
Research Use
For research use only. Not for human or drug use

Figure 1 Example of fluorometric standard curve using Glycogen Assay Kit.


Figure 2 Measurement of glycogen in various mouse tissues using Glycogen Assay Kit.


Figure 3 Glycogen concentration measured in MBA-MB-231 cells (human breast adenocarcinoma cell line). 106 cells were prepared following protocol instructions, and several dilutions were measured using fluorometric detection.

All products and services are for Research Use Only. Do Not use in humans.

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