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Fructose Assay Kit (Colorimetric/Fluorometric) (CAT#: CB-P162-K) Datasheet

Product Type
Kit
Description
In Creative Biolabs' fructose assay kit, free fructose is converted into β-glucose by enzymes, and then specifically converted into products that react with OxiRed Probe to generate color (λ = 570nm) and Fluorometric (Ex / Em = 535 / 587nm). The kit provides a fast, simple, sensitive and reliable for high-throughput assay of D-fructose. Do not use this kit with urine, serum or plasma samples.
Applications
Functional Studies
Size
1 kit
Detection Method
Colorimetric/Fluorometric
Sample
Cell culture extracts, Tissue Extracts
Species Reactivity
Mammals
Protocol Length
1h 00m
Components
Enzyme Mix (lyophilized) 1 vial
Fructose Assay Buffer 1 x 25ml
Fructose Converting Enzyme (Lyophilised) 1 vial
Fructose Standard (100mM) 1 x 100µl
OxiRed Probe in DMSO 1 x 200µl
Requires
Microplate reader
Storage
Please refer to protocols.
Shelf Life
12 months
Research Use
For research use only. Not for human or drug use

Figure 1 Fructose levels in mouse liver and banana, determined using the colorimetric method and presented per mg of protein (duplicates; +/- SD).


Figure 2 Titration of the fructose (background subtracted, duplicates; +/- SD).


Figure 3 Fructose levels banana, determined using the colorimetric (C) and the fluorometric (F) method (presented per mg of protein) (duplicates; +/- SD).

All products and services are for Research Use Only. Do Not use in humans.

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