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Free Glycerol Assay Kit (Colorimetric) (CAT#: CB-P183-K) Datasheet

Product Type
Kit
Description
Creative Biolabs' Free Glycerin Assay Kit II is suitable for measuring the level of free glycerin in samples containing reducing substances, which may interfere with oxidase-based assays. In this assay, glycerol is converted into an intermediate in the presence of a glycerase mixture, which reduces the colorless probe to a colored product with strong absorption at 450 nm. The free glycerol test kit II is simple, fast and has high throughput adaptability. The assay kit can detect free glycerol less than 20μM in various biological samples.
Applications
Functional Studies
Size
1 kit
Detection Method
Colorimetric/Fluorometric
Detection Limit
2 nmol/well - 10 nmol/well
Sensitivity
< 20 µM
Sample
Cell culture supernatant, Urine, Serum, Plasma, Tissue, Adherent cells, Suspension cells, Tissue Homogenate
Species Reactivity
Mammals
Protocol Length
2h 00m
Components
Glycerol Assay Buffer 1 x 25ml
Glycerol Enzyme Mix (lyophilized) 1 vial
Glycerol Probe 1 vial
Glycerol Standard 1 x 0.2ml
Requires
Microplate reader
Storage
Please refer to protocols.
Shelf Life
12 months
Research Use
For research use only. Not for human or drug use

Figure 1 Glycerol Standard Curve [a]. Measurement of free glycerol in bovine liver (50 µg), Jurkat cells (40 µg), HeLa cells (30 µg), fish egg (58 µg) & human serum (2 µl) [b].

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All products and services are for Research Use Only. Do Not use in humans.

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