Malate Assay Kit (Colorimetric) (CAT#: CB-P187-K) Datasheet

Product Type
Kit
Description
Creative Biolabs' Malic Acid assay Kit is a simple and sensitive assay that can measure the level of malic acid L(-) in a variety of samples. In the assay, malic acid is specifically oxidized to produce a product, which reacts with the substrate probe to produce a color (λmax = 450 nm). This assay can detect 1-10 nmol of malic acid in a 50 µl sample, with a assay sensitivity of about 20 µM.
Applications
Functional Studies
Size
1 kit
Detection Method
Colorimetric/Fluorometric
Detection Limit
20 µM - 200 µM
Sensitivity
20 µM
Sample
Cell culture supernatant, Urine, Serum, Plasma, Tissue, Adherent cells, Suspension cells, Tissue Homogenate
Species Reactivity
Mammals
Protocol Length
1h 00m
Components
Malate assay buffer 1 x 20ml
Malate Enzyme Mix (Lyophilised) 1 vial
Malate Standard (10 µmol) (Lyophilized) 1 vial
WST Substrate (Lyophilised) 1 vial
Requires
Microplate reader
Storage
Please refer to protocols.
Shelf Life
12 months
Research Use
For research use only. Not for human or drug use

Figure 1 Example of Standard Curve

All products and services are for Research Use Only. Do Not use in humans.

ONLINE INQUIRY

Creative Biolabs has established a team of customer support scientists ready to discuss ADCC/CDC optimization strategies, antibody production, bioinformatics analysis and other molecular biology/biotechnology issues.

  • *
  • *
  • *
USA

UK