Product Type
Kit
Description
The plate is a 96-well membrane bottom plate, which contains a special modified polyethersulfone membrane with a molecular weight cut-off of approximately 30 kDa.
Features
Number of Samples: Sufficient for up to 96 samples.
Amount of Sample: Up to 350 µL per well
Centrifugal Force 1500 x g
Operating Vacuum 10-20 in Hg (approx. 0.35-0.7 bar)
Suitable Samples: Unlabelled and fluorophore labelled (e.g. 2-AB, 2-AA or procainamide labelled) glycans
released from glycoproteins or other sources and treated with exoglycosidase
enzymes.
Applications
Post-exoglycosidase Clean-up
For removal of exoglycosidase enzymes and other protein material following glycan enzymatic digestion/ sequencing. This will prevent contamination of HPLC columns during subsequent chromatographic analysis. The plate can also be used to remove exoglycosidases or other proteins before mass spectrometry analysis of glycans.
Sample
Glycoproteins and glycopeptides containing N-linked glycans
Components
CreaClean 96-well Post-Exoglycosidase Clean-up Plate 1
Handling Advice
Ensure that any glass, plasticware or solvents used are free of glycosidases and environmental carbohydrates. Use powder-free gloves for all sample handling procedures and avoid contamination with environmental carbohydrate. Once individual vials of reagents are opened, their contents should be used immediately. Discard any excess according to local safety rules.
Storage
Store at -18°C in the dark. Protect from sources of heat, light, and moisture. The reagents are stable for at least two years as supplied.
Shelf Life
Stability: Stable at least 12 months when stored properly. Several days exposure to ambient tempertures will not reduce activity.
Research Use
For research use only. Not for human or drug use
