Product Type
Kit
Description
The GlycoProfile™ Enzymatic In-gel N-Deglycosylation Kit is optimized to provide a convenient, reproducible to N-deglycosylate and digest protein samples from one dimensional (1D) and two dimensional (2D) polyacrylamide gel pieces for subsequent mass spectrometry or HPLC analysis.
Applications
Each kit contains reagents sufficient to deglycosylate and digest up to 10 samples.
Principle
The kit includes Proteomics Grade PNGase F and trypsin enzymes necessary for N-linked deglycosylation and tryptic digestion, respectively. The samples can then be desalted and concentrated for analysis by MALDI-TOF MS or electrospray MS with subsequent database searching.
Size
1 kit
Detection Method
Colorimetric/Fluorometric
Sample
Cell culture supernatant, Urine, Serum, Plasma, Tissue, Adherent cells, Suspension cells, Tissue Homogenate
Protocol Length
1h 00m
Components
PNGase F 50 U
Trypsin from porcine pancreas, Dimethylated 20 μg
Trypsin Solubilization Reagent
Invertase Glycoprotein Standard
Acetonitrile, ≥99.93%, 50 mL
Requires
Table-top shaker with heating unit.
Centrifuge suitable for 2 mL Eppendorf tubes (floor or table-top).
Centrifugal evaporator suitable for 2 mL Eppendorf tubes.
HPLC grade acetonitrile (1 mL). Do not use lower purity.
Acetic acid (1 mL).
Storage
2-8°C
Shelf Life
≥1 yr at 2‑8 °C
Research Use
For research use only. Not for human or drug use
