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CreaTag™ Quantitative Sialic Acid Release and DMB Labelling Kit [For Glycan Labeling] (CAT#: CB-P285-K) Datasheet

Product Type
Kit
Description
The kit contains reagents for releasing sialic acid from glycoproteins. The released sialic acid is conjugated to the DMB dye through an amination-cyclization reaction.
Features
Dye PropertiesRelative Molecular Mass = 225.07 gmol-1 Fluorescence, λex = 373 nm, λem = 448 nm.
Synonyms DMB; 1,2-Diamino-4,5-methylenedioxybenzene Dihydrochloride; 1,3-Benzodioxole5,6-diamine Dihydrochloride; 5,6-Diamino-1,3-benzodioxole Dihydrochloride
Number of Samples: The kit contains reagents and materials for up to 22 samples including the sialic acid
reference panel, and the N-acetyl neuraminic acid and N-glycolyl neuraminic acid
quantitative standards.
Amount of Sample: Typically start with 50-200 µg of glycoprotein per analysis. We recommend analysing
samples in triplicate.
Suitable Samples: Any sialic acid released from a glycoprotein, glycopeptide or glycan can be labelled.
Applications
For release of sialic acids from glycoproteins and labeling with 1,2-diamino-4,5- methylenedioxybenzene.2HCl (DMB).
Sample
Glycoproteins and glycopeptides containing N-linked glycans
Components
DMB Dye 0.7 mg
Acetic Acid 2 Molar 2 x 1.1 mL
Mercaptoethanol in Acetic acid (1.4 Molar) 500 µL
Sodium Dithionite (Reductant) 4 mg
N-acetylneuraminic acid quantitative standard 1 nmol
N-glycolylneuraminic acid quantitative standard 1 nmol
Sialic Acid Reference Panel containing Neu5Ac, Neu5Gc,Neu5,7Ac2, Neu5Gc,9Ac, Neu5,8Ac2, Neu5,9Ac2 and Neu5,x,xAc3 (where x is an unknown acetyl position). (total sialic acids) 1.25 nmol
Handling Advice
Ensure that any glass, plasticware or solvents used are free of glycosidases and environmental carbohydrates. Use powder-free gloves for all sample handling procedures and avoid contamination with environmental carbohydrate. Once individual vials of reagents are opened, their contents should be used immediately. Discard any excess according to local safety rules.
Storage
Store at -18°C in the dark. Protect from sources of heat, light, and moisture. The reagents are stable for at least two years as supplied.
Shelf Life
Stability: Stable at least 12 months when stored properly. Several days exposure to ambient tempertures will not reduce activity.
Research Use
For research use only. Not for human or drug use

Figure 1 Chromatogram of DMB Labelled Sialic Acid Reference Panel (CM-SRP-01) run on the HPLC column.

Figure 2 DMB Labelled Sialic Acid Reference Panel run on the HPLC column.

Figure 3 Reaction Mechanism.

Related Products

CreaClean™ Pre-Permethylation Clean-up Plate [For Glycan Clean Up] (CAT#: CB-P281-K)
NA2 Glycan (A2G2, G2), 2-AB Labelled [Labeled Glycan Standards] (CAT#: CB-P337-K)
NGA4 Glycan (A4), 2-AA Labelled [Labeled Glycan Standards] (CAT#: CB-P356-K)
Recombinant PNGase F [For Glycan Release] (CAT#: CB-P251-K)
CreaZyme™ Ceramide Glycanase Kit [For Glycan Release] (CAT#: CB-P262-K)
N-glycan IgG library, permethylated with heavy (13C) MeI [Permethylated Glycan Standards] (CAT#: CB-P321-K)
Endo-β-Galactosidase [For Glycan Release] (CAT#: CB-P261-K)
M3N2 Glycan (Man-3), 2-AB Labelled [Labeled Glycan Standards] (CAT#: CB-P339-K)

All products and services are for Research Use Only. Do Not use in humans.

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