Connect with Us at the Upcoming Events

Creative Biolabs is excited to greet you at the upcoming international conferences. Meet our team at the 13th Annual World ADC San Diego and the 13th Annual World Multispecific Summit in September (booth number to be updated) for expert consultation on your drug discovery. Shoot an email to arrange an in-person meeting!

Book a Meeting

CreaTag™ Quantitative Sialic Acid Release and DMB Labelling Kit [For Glycan Labeling] (CAT#: CB-P285-K) Datasheet

Product Type
The kit contains reagents for releasing sialic acid from glycoproteins. The released sialic acid is conjugated to the DMB dye through an amination-cyclization reaction.
Dye PropertiesRelative Molecular Mass = 225.07 gmol-1 Fluorescence, λex = 373 nm, λem = 448 nm.
Synonyms DMB; 1,2-Diamino-4,5-methylenedioxybenzene Dihydrochloride; 1,3-Benzodioxole5,6-diamine Dihydrochloride; 5,6-Diamino-1,3-benzodioxole Dihydrochloride
Number of Samples: The kit contains reagents and materials for up to 22 samples including the sialic acid
reference panel, and the N-acetyl neuraminic acid and N-glycolyl neuraminic acid
quantitative standards.
Amount of Sample: Typically start with 50-200 µg of glycoprotein per analysis. We recommend analysing
samples in triplicate.
Suitable Samples: Any sialic acid released from a glycoprotein, glycopeptide or glycan can be labelled.
For release of sialic acids from glycoproteins and labeling with 1,2-diamino-4,5- methylenedioxybenzene.2HCl (DMB).
Glycoproteins and glycopeptides containing N-linked glycans
DMB Dye 0.7 mg
Acetic Acid 2 Molar 2 x 1.1 mL
Mercaptoethanol in Acetic acid (1.4 Molar) 500 µL
Sodium Dithionite (Reductant) 4 mg
N-acetylneuraminic acid quantitative standard 1 nmol
N-glycolylneuraminic acid quantitative standard 1 nmol
Sialic Acid Reference Panel containing Neu5Ac, Neu5Gc,Neu5,7Ac2, Neu5Gc,9Ac, Neu5,8Ac2, Neu5,9Ac2 and Neu5,x,xAc3 (where x is an unknown acetyl position). (total sialic acids) 1.25 nmol
Handling Advice
Ensure that any glass, plasticware or solvents used are free of glycosidases and environmental carbohydrates. Use powder-free gloves for all sample handling procedures and avoid contamination with environmental carbohydrate. Once individual vials of reagents are opened, their contents should be used immediately. Discard any excess according to local safety rules.
Store at -18°C in the dark. Protect from sources of heat, light, and moisture. The reagents are stable for at least two years as supplied.
Shelf Life
Stability: Stable at least 12 months when stored properly. Several days exposure to ambient tempertures will not reduce activity.
Research Use
For research use only. Not for human or drug use

Figure 1 Chromatogram of DMB Labelled Sialic Acid Reference Panel (CM-SRP-01) run on the HPLC column.

Figure 2 DMB Labelled Sialic Acid Reference Panel run on the HPLC column.

Figure 3 Reaction Mechanism.

All products and services are for Research Use Only. Do Not use in humans.


Creative Biolabs has established a team of customer support scientists ready to discuss ADCC/CDC optimization strategies, antibody production, bioinformatics analysis and other molecular biology/biotechnology issues.

  • *
  • *
  • *