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DeGlyco™ Native Antibody/Protein Deglycosylation Kit (CAT#: CB-P196-K) Datasheet

Product Type
Kit
Description
All complex oligosaccharides can be reduced to the trimannosylchitobiose core by treatment of the glycoproteins with neuraminidase, β-galactosidase, and N-acetylglucosaminidase. Fucosidase may be required in some cases. The remaining trimannosylchitobiose core The sugar core structure can be removed by endoglycosidase F3. Endoglycosidases F2 and F3 can immediately remove the triantenna structure, and endoglycosidase F1 can remove oligomannose and hybrid structures.

Natural protein deglycosylation is designed to remove N-linked oligosaccharides from PNGase F-resistant natural proteins. Endoglycosidase F1, F2 and F3 are not as sensitive to protein conformation as PNGase F, and are more suitable for removing all types of N-linked oligosaccharides without protein denaturation.
Applications
Glycosylation Kit has been for N-deglycosylation of various enzymes such as laccase, tomato nuclease TBN1 and endo-β-1,3-glucanase.
Size
1 kit
Detection Method
Colorimetric/Fluorometric
Sample
Cell culture supernatant, Urine, Serum, Plasma, Tissue, Adherent cells, Suspension cells, Tissue Homogenate
Species Reactivity
Mammals
Protocol Length
1h 00m
Components
Endoglycosidase F1.3 U
Endoglycosidase F2.1 U
Endoglycosidase F3.1 U
Endoglycosidase F1 reaction buffer 200 μL
Endoglycosidase F2 & 3 reaction buffer 200 μL
Requires
Table-top shaker with heating unit.
Centrifuge suitable for 2 mL Eppendorf tubes (floor or table-top).
Centrifugal evaporator suitable for 2 mL Eppendorf tubes.
HPLC grade acetonitrile (1 mL). Do not use lower purity.
Acetic acid (1 mL).
Storage
2-8°C
Shelf Life
≥1 yr at 2‑8 °C
Research Use
For research use only. Not for human or drug use

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All products and services are for Research Use Only. Do Not use in humans.

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