Complement Complex (TCC) ELISA Kit [Rat]-1 x 96 det. (CAT#: CB-P081-K) Datasheet

Kit

Three different activation pathways of complement are fused with the formation of C5 invertase, and it is the cleavage of C5 by these invertases that initiates the cleavage or terminal pathway. In contrast to activation pathways that require enzymatic cleavage, the terminal pathway relies on binding-induced conformational changes. The binding of C6 promotes the binding of C7, which changes the conformation of the complex. After C8 is bound, a variable number of C9 molecules bind to the C5b678 complex (the so-called terminal complement complex (TCC) or membrane attack complex (MAC)). The formation of TCC membrane form can cause cell lysis, or trigger a variety of cellular metabolic pathways, leading to the synthesis and release of inflammatory mediators. TCC contains neoantigens that are not present in a single natural component. Neoantigens exist in both membrane-bound (MAC) and liquid phase (SC5b-9) complexes. TCC is present in normal human plasma, <1 AU/ml, and increases in patients with complement activation.

The rat terminal complement complex (TCC) ELISA kit is used for in vitro quantitative determination of rat TCC in serum and plasma samples.

Rat TCC ELISA kit is a ready-to-use solid-phase enzyme-linked immunosorbent assay based on the sandwich principle. The working time is 3 hours. The effective form of the plate with twelve disposable 8-well plates allows free choice of batch size for measurement. The samples and standards are incubated in microtiter wells coated with antibodies that recognize rat TCC. The biotinylated tracer antibody will bind to the captured rat TCC. The streptavidin-peroxidase conjugate will bind the biotinylated tracer antibody. The streptavidin-peroxidase conjugate will react with the substrate tetramethylbenzidine (TMB). The enzyme reaction is stopped by adding oxalic acid. Measure the absorbance at 450nm with a spectrophotometer. Draw a standard curve by plotting the absorbance (linear) against the corresponding concentration (logarithm) of the rat TCC standard. The concentration of the rat TCC sample running simultaneously with the standard can be determined from the standard curve.