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Superoxide Dismutase (SOD) Activity Colorimetric Assay Kit (CAT#: CB-P053-K) Datasheet

Description
Superoxide dismutase (SOD) is one of the most important antioxidant enzymes. It catalyzes the disproportionation of superoxide anions into hydrogen peroxide and molecular oxygen. The sensitive SOD analysis kit uses WST-1, which can produce water-soluble maz dye after reduction with superoxide anion. The reduction rate of superoxide anion is linearly related to xanthine oxidase (XO) activity. Therefore, the inhibitory activity of SOD can be determined by colorimetry.
Features
Simple one-step procedure; takes around than 30 minutes
Fast and convenient
Applications
Kit contains the necessary reagents for convenient measurement of activity of Superoxide Dismutase (SOD) by colorimetric method.
Size
100 assays
Detection Method
Absorbance (450 nm)
Sample
Whole blood, plasma, serum, urine, tissue and cell extracts
Species Reactivity
Mammalian
Components
WST Solution
SOD Enzyme Solution
SOD Assay Buffer
SOD Dilution Buffer
Shipping
Gel Pack
Storage
4 °C.
Shelf Life
24 months from date of purchase, if stored properly.
Research Use
For Research Use Only! Not For Use in Humans.

Figure: SOD Activity (% inhibition rate): human serum (10 µl) and isolated mitochondria from Jurkat cells (10 µg), and yeast (Saccromyces cerevisiae, 100 ug), was used to determine SOD Activity according to the kit protocol. Activity was measured in 15 min. at 37°C.

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All products and services are for Research Use Only. Do Not use in humans.

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