ADCC Assay Kit (CAT#: CB-P001-K) Datasheet

Description
Cell-mediated cytotoxicity is an important phenomenon characterized by the immune system causing damaged cells to be lysed in the body. The activation of the immune system results in the removal of target cells infected by pathogens or transformed cells/cancer cells. This process is mediated by antibody-dependent cell-mediated cytotoxicity (ADCC), complement-mediated cytotoxicity, or lymphocyte-mediated cytotoxicity. Our cell-mediated cytotoxicity analysis kit contains carboxyfluorosuccinimide ester (CFSE) (a green fluorescent probe for labeling live target cells) and 7-aminoactinomycin D (7-AAD), a red fluorescent probe that marks late apoptotic and necrotic target cells killed in cancer cells. The assay does not require cell lysis, and can directly measure cytotoxicity rather than indirect indicators such as ATP release or lactate dehydrogenase activity. The method based on flow cytometry can provide reliable data and support multi-parameter analysis.
Applications
Measuring cytotoxicity in response to drug or toxin treatment
Quantification of the cytotoxic effects of immune effector cells on target cells
Evaluation of physiological mediators and antibodies that affect cell cytotoxicity
Size
100 assays
Detection Method
Fluorescence (FL1 channel and FL3 channel)
Sample
Cells etc
Species Reactivity
All species
Components
Cytotoxicity Assay Buffer
7-AAD Staining Solution
CFSE Staining Solution
Shipping
Gel Pack
Storage
-20°C
Shelf Life
12 months
Research Use
For Research Use Only! Not For Use in Humans.

Figure: Cytotoxicity assay kit: Jurkat cells (105 cells/ml) are grown in RPMI medium supplemented with 10% FBS. Treat the cells with camptothecin (5 µM) overnight. The next day, the cells were stained with CFSE and 7-AAD for 30 minutes. At 37°C. The figure (right) shows the cytotoxic effect of the compound and illustrates the apoptosis of cells using CFSE and 7-AAD.

All products and services are for Research Use Only. Do Not use in humans.

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Creative Biolabs has established a team of customer support scientists ready to discuss ADCC/CDC optimization strategies, antibody production, bioinformatics analysis and other molecular biology/biotechnology issues.

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