ADCC Assay Kit (CAT#: CB-P001-K) Datasheet

Cell-mediated cytotoxicity is an important phenomenon characterized by the immune system causing damaged cells to be lysed in the body. The activation of the immune system results in the removal of target cells infected by pathogens or transformed cells/cancer cells. This process is mediated by antibody-dependent cell-mediated cytotoxicity (ADCC), complement-mediated cytotoxicity, or lymphocyte-mediated cytotoxicity. Our cell-mediated cytotoxicity analysis kit contains carboxyfluorosuccinimide ester (CFSE) (a green fluorescent probe for labeling live target cells) and 7-aminoactinomycin D (7-AAD), a red fluorescent probe that marks late apoptotic and necrotic target cells killed in cancer cells. The assay does not require cell lysis, and can directly measure cytotoxicity rather than indirect indicators such as ATP release or lactate dehydrogenase activity. The method based on flow cytometry can provide reliable data and support multi-parameter analysis.

Measuring cytotoxicity in response to drug or toxin treatment
Quantification of the cytotoxic effects of immune effector cells on target cells
Evaluation of physiological mediators and antibodies that affect cell cytotoxicity