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Bradford Protein Assay (CAT#: CB-P005-K) Datasheet

The Bradford protein assay is based on the modified Coomassie Blue G method. The dye forms a blue complex with protein, and the color intensity measured at 595nm is directly proportional to the protein concentration in the sample. Our optimized formulation can greatly reduce the interference of substances in the original sample and show higher sensitivity to peptides.
• Sensitive and accurate. Use 10 mL sample. The detection range in 96-well plate analysis is 0.06-1.0 mg/mL protein.
• Simple and high throughput. The "mix and read" procedure involves adding a single working reagent and reading the optical density. High-throughput analysis can be easily automated in 96-well plates, and thousands of samples can be processed every day.
• Low interference. Glucose, Tris, vitamins and amino acids, DNA, RNA, salt, EDTA (<12 mM), phenol (<50 mM), urea (<0.6 M), Triton (<0.1%) and SDS (<0.1% SDS ) do not interfere with the measurement.
• Versatility: It can be measured in 96-well plates or cuvettes.
For quantitative determination of total protein.
500 tests
Detection Method
OD595nm (Bradford)
Detection Limit
60 ug/mL
Biological, food, agriculture etc
Species Reactivity
All species
Protocol Length
2 min
Reagent: 20 mL 5 x concentrate Protein standard: 1 mL 1.0 mg/mL BSA
Gel Pack
Store the reagent at 4°C and standard at -20°C
Shelf Life
12 months
Research Use
For Research Use Only! Not For Use in Humans.

All products and services are for Research Use Only. Do Not use in humans.


Creative Biolabs has established a team of customer support scientists ready to discuss ADCC/CDC optimization strategies, antibody production, bioinformatics analysis and other molecular biology/biotechnology issues.

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