Description
The Bradford protein assay is based on the modified Coomassie Blue G method. The dye forms a blue complex with protein, and the color intensity measured at 595nm is directly proportional to the protein concentration in the sample. Our optimized formulation can greatly reduce the interference of substances in the original sample and show higher sensitivity to peptides.
Features
• Sensitive and accurate. Use 10 mL sample. The detection range in 96-well plate analysis is 0.06-1.0 mg/mL protein.
• Simple and high throughput. The "mix and read" procedure involves adding a single working reagent and reading the optical density. High-throughput analysis can be easily automated in 96-well plates, and thousands of samples can be processed every day.
• Low interference. Glucose, Tris, vitamins and amino acids, DNA, RNA, salt, EDTA (<12 mM), phenol (<50 mM), urea (<0.6 M), Triton (<0.1%) and SDS (<0.1% SDS ) do not interfere with the measurement.
• Versatility: It can be measured in 96-well plates or cuvettes.
Applications
For quantitative determination of total protein.
Size
500 tests
Detection Method
OD595nm (Bradford)
Detection Limit
60 ug/mL
Sample
Biological, food, agriculture etc
Protocol Length
2 min
Components
Reagent: 20 mL 5 x concentrate Protein standard: 1 mL 1.0 mg/mL BSA
Shipping
Gel Pack
Storage
Store the reagent at 4°C and standard at -20°C
Shelf Life
12 months
Research Use
For Research Use Only! Not For Use in Humans.
